Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Gallus GREM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Gallus Gremlin 1 (GREM1) Polyclonal Antibody | anti-GREM1 antibody
Polyclonal Antibody to Gremlin 1 (GREM1)
Gene Names
GREM1; Drm
Reactivity
Gallus
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Gremlin 1 (GREM1); Polyclonal Antibody; Polyclonal Antibody to Gremlin 1 (GREM1); anti-GREM1 antibody
Host
Rabbit
Reactivity
Gallus
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GREM1. It has been selected for its ability to recognize GREM1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.59mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-RNRG SQGAIPPPDK DQPNDSEQMQ TQQQSGSRHR ERGKGTSMPA EEVLESSQEA LHITERKYLK RDWCKTQPLK QTIHEEGCNS RTIINRFCYG QCNSFYIPRH VRKEEGSFQS CSFCKPKKFT TMTVTLNCPE LQPPRKKKRI TRVKECRCIS IDLD
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-RNRG SQGAIPPPDK DQPNDSEQMQ TQQQSGSRHR ERGKGTSMPA EEVLESSQEA LHITERKYLK RDWCKTQPLK QTIHEEGCNS RTIINRFCYG QCNSFYIPRH VRKEEGSFQS CSFCKPKKFT TMTVTLNCPE LQPPRKKKRI TRVKECRCIS IDLD
Sequence Length
184
Applicable Applications for anti-GREM1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GREM1 (Arg27~Asp184) expressed in E.coli.
Cross Reactivity
Mouse, Chicken
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2063397
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Gallus GREM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Gallus GREM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,166 Da
NCBI Official Full Name
gremlin-1
NCBI Official Synonym Full Names
gremlin 1, DAN family BMP antagonist
NCBI Official Symbol
GREM1
NCBI Official Synonym Symbols
Drm
NCBI Protein Information
gremlin-1
UniProt Protein Name
Gremlin-1
Protein Family
UniProt Gene Name
GREM1
Uniprot Description
Cytokine that may play a role in the development of the medial pallium and during optic nerve and pecten development by modulating BMP signaling.
Research Articles on GREM1
Similar Products
Product Notes
The GREM1 grem1 (Catalog #AAA2006097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Gremlin 1 (GREM1) reacts with Gallus and may cross-react with other species as described in the data sheet. AAA Biotech's Gremlin 1 (GREM1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GREM1 grem1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-RNRG SQGAIPPPDK DQPNDSEQMQ TQQQSGSRHR ERGKGTSMPA EEVLESSQEA LHITERKYLK RDWCKTQPLK QTIHEEGCNS RTIINRFCYG QCNSFYIPRH VRKEEGSFQS CSFCKPKKFT TMTVTLNCPE LQPPRKKKRI TRVKECRCIS IDLD. It is sometimes possible for the material contained within the vial of "Gremlin 1 (GREM1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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