Western Blot (WB)
(Western Blot: Sample: Mouse Thymus lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse GRAP2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse GRB2 Related Adaptor Protein 2 (GRAP2) Polyclonal Antibody | anti-GRAP2 antibody
Polyclonal Antibody to GRB2 Related Adaptor Protein 2 (GRAP2)
Gene Names
Grap2; P38; GRID; Gads; GrbX; GrpL; Mona; GRB2L; GRBLG; Grf40; GRAP-2
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
GRB2 Related Adaptor Protein 2 (GRAP2); Polyclonal Antibody; Polyclonal Antibody to GRB2 Related Adaptor Protein 2 (GRAP2); anti-GRAP2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GRAP2. It has been selected for its ability to recognize GRAP2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.87mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-R TGDILKILSN QEEWLKAELG SQEGYVPKNF IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGLGSEVNATLMH RRHTDPVQLQ AA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-R TGDILKILSN QEEWLKAELG SQEGYVPKNF IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGLGSEVNATLMH RRHTDPVQLQ AA
Sequence Length
330
Applicable Applications for anti-GRAP2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GRAP2 (Arg20~Ala262) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2080666
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Thymus lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse GRAP2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Thymus lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse GRAP2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,810 Da
NCBI Official Full Name
GRB2-related adaptor protein 2
NCBI Official Synonym Full Names
GRB2-related adaptor protein 2
NCBI Official Symbol
Grap2
NCBI Official Synonym Symbols
P38; GRID; Gads; GrbX; GrpL; Mona; GRB2L; GRBLG; Grf40; GRAP-2
NCBI Protein Information
GRB2-related adaptor protein 2
UniProt Protein Name
GRB2-related adaptor protein 2
Protein Family
UniProt Gene Name
Grap2
UniProt Synonym Gene Names
Gads; Grb2l; Grid; Mona; GRB2L; MONA; Monocytic adapter
Uniprot Description
GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 15|15 E1
Cellular Component: cytoplasm; endosome; extrinsic component of cytoplasmic side of plasma membrane; nucleus
Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; receptor binding
Biological Process: cell differentiation; cell migration; innate immune response; peptidyl-tyrosine autophosphorylation; regulation of cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on GRAP2
Similar Products
Product Notes
The GRAP2 grap2 (Catalog #AAA2002506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to GRB2 Related Adaptor Protein 2 (GRAP2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GRB2 Related Adaptor Protein 2 (GRAP2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GRAP2 grap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-R TGDILKILSN QEEWLKAELG SQEGYVPKNF IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL GSEVNATLMH RRHTDPVQLQ AA. It is sometimes possible for the material contained within the vial of "GRB2 Related Adaptor Protein 2 (GRAP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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