Goat GRB2 Polyclonal Antibody | anti-GRB2 antibody

Goat anti-GRB2 Antibody

Cat. Num. AAA421084

• Gene Names: GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
• Reactivity: Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GRB2; Polyclonal Antibody; Goat anti-GRB2 Antibody; growth factor receptor-bound protein 2; ASH; ASH protein; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; HT027; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; growth factor receptor-bound protein 3; NCKAP2; OTTHUMP00000166096; GRB2 antibody; growth factor receptor-bound protein 2 antibody; ASH antibody; ASH protein antibody; EGFRBP-GRB2 antibody; Grb3-3 antibody; MST084 antibody; MSTP084 antibody; HT027 antibody; abundant SRC homology antibody; epidermal growth factor receptor-binding protein GRB2 antibody; growth factor receptor-bound protein 3 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; anti-GRB2 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PRNYVTPVNRNV
• Sequence Length: 217

• Applicable Applications for anti-GRB2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Data obtained by previous batch (different goat). Recommended concentration, 0.1-0.3ug/ml. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained.; Western Blot: Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15ug/ml.
• Immunogen: Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.
• Epitope: C Terminus
• Note: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB)

((0. 03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0. 05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Immunohistochemistry (IHC)

((0.3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504111
• NCBI GeneID: 2885
• NCBI Accession #: NP_002077.1; NP_987102.1
• NCBI GenBank Nucleotide #: NM_002086.4
• Molecular Weight: 20,557 Da
• NCBI Official Full Name: growth factor receptor-bound protein 2 isoform 1
• NCBI Official Synonym Full Names: growth factor receptor bound protein 2
• NCBI Official Symbol: GRB2
• NCBI Official Synonym Symbols: ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
• NCBI Protein Information: growth factor receptor-bound protein 2
• UniProt Protein Name: Growth factor receptor-bound protein 2
• Protein Family: GRB2-related adapter protein
• UniProt Gene Name: GRB2
• UniProt Synonym Gene Names: ASH
• UniProt Entry Name: GRB2_HUMAN

NCBI Description

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.