Rabbit anti-Human GRB10 Polyclonal Antibody | anti-GRB10 antibody

GRB10

Cat. Num. AAA617265

• Gene Names: GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10; KIAA0207
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: GRB10; Polyclonal Antibody; Anti -GRB10; anti-GRB10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human total GRB10 protein independent of phosphorylation at 76kD. Does not crossreact with GRB7 or GRB14.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-GRB10 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to N-terminal residues of human GRB10 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HEK293, SK-N-MC, COS and Grb10 Wild-type transfected COS cells, using MBS617265.)

Testing Data

Related Product Information for anti-GRB10 antibody

GRB7 family adaptor proteins consist of GRB7, GRB10 and GRB14, which contain an N-terminal proline-rich SH3 binding domain, followed by PH, PBS and SH2 domains. Each member of the family has several splicing isoforms (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules such as Raf, Akt and Nedd4 (1,2). Although it was originally reported that GRB10 is exclusively phosphorylated at serine residues (3), recent research has shown that GRB10 is also phosphorylated at tyrosine 67 by Src kinase family members (4).

Product Categories/Family for anti-GRB10 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 48762697
• NCBI GeneID: 2887
• NCBI Accession #: NP_001001555.1
• NCBI GenBank Nucleotide #: NM_001001555.2
• UniProt Accession #: O00428; Q13322; Q75M93; Q75MT1
• Molecular Weight: 12,393 Da
• NCBI Official Full Name: growth factor receptor-bound protein 10 isoform c
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 10
• NCBI Official Symbol: GRB10
• NCBI Official Synonym Symbols: RSS; IRBP; MEG1; GRB-IR; Grb-10; KIAA0207
• NCBI Protein Information: growth factor receptor-bound protein 10; OTTHUMP00000197988; OTTHUMP00000197989; OTTHUMP00000197990; OTTHUMP00000197991; OTTHUMP00000197992; OTTHUMP00000197993; OTTHUMP00000197994; GRB10 adapter protein; GRB10 adaptor protein; maternally expressed gene 1; insulin receptor-binding protein Grb-IR
• UniProt Protein Name: Adapter protein
• Protein Family: Growth factor receptor-bound protein
• UniProt Entry Name: O00428_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Uniprot Description

GRB10: an adaptor protein that inhibits insulin-stimulated IRS-PI (3)K-Akt signaling pathway by disrupting the association of IRS with the insulin receptor. Also binds activated PDGFR and EGFR. Preferentially localizes to the mitochondria, modulating the anti-apoptotic activity of mitochondrial Raf-1. Can translocate to the plasma membrane and lamellipodia. Three alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; insulin receptor binding

Biological Process: negative regulation of glucose import; negative regulation of Wnt receptor signaling pathway; insulin-like growth factor receptor signaling pathway; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphorylation; response to insulin stimulus; negative regulation of glycogen biosynthetic process