Rabbit anti-Human Granzyme B Polyclonal Antibody | anti-GZMB antibody

Polyclonal Antibody to Granzyme B (GZMB)

Cat. Num. AAA2027995

• Gene Names: GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Granzyme B; Polyclonal Antibody; Polyclonal Antibody to Granzyme B (GZMB); anti-GZMB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against GZMB. It has beenselected for its ability to recognize GZMB in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IIGGHEAKPH SRPYMAYLMI WDQKSLKRCG GFLIRDDFVL TAAHCWGSSI NVTLGAHNIK EQEPTQQFIP VKRPIPHPAY NPKNFSNDIM LLQLERKAKR TRAVQPLRLP SNKAQVKPGQ TCSVAGWGQT APLGKHSHTL QEVKMTVQED RKCESDLRHY YDSTIELCVG DPEIKKTSFK GDSGGPLVCN KVAQGIVSYG
• Sequence Length: 247

• Applicable Applications for anti-GZMB antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-2ug/ml; Immunocytochemistry in formalin fixed cells: 5-20ug/ml; Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml; Immunohistochemistry in paraffin section: 5-20ug/ml; Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml; Optimal working dilutions must be determined by end user.
• Fragment: GZMB (Ile21~Tyr247)
• Organism Species: Homo sapiens (Human)
• Cross Reactivity: Human, Mouse
• Conjugate: No Conjugate
• Immunogen: Recombinant GZMB (Ile21~Tyr247) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2044794
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Thymus Tissue; Lane2: Mouse Lymph Node Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant GZMB, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Brain Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast cancer Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122.2
• NCBI GenBank Nucleotide #: NM_004131.5
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27,716 Da
• NCBI Official Full Name: granzyme B isoform 1 preproprotein
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Apoptosis; EC 3.4.21.79; Protease

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; immunological synapse; membrane; mitochondrion; nucleus; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: apoptosis; cytolysis; induction of apoptosis by granzyme; natural killer cell mediated cytotoxicity; proteolysis