Immunohistochemistry (IHC)
(Immunochemical staining of mouse GRN in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse Granulin Polyclonal Antibody | anti-GRN antibody
Anti-Granulin Antibody, Rabbit Polyclonal
Gene Names
Grn; Pgrn; epithelin
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Granulin; Polyclonal Antibody; Anti-Granulin Antibody; Rabbit Polyclonal; Granulin/GRN/Progranulin Antibody; Rabbit PAb; Antigen Affinity Purified; Granulin Precursor; Anti-epithelin Antibody; Anti-Pgrn Antibody; anti-GRN antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse GRN/Granulin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GRN antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:1000-1:2000
IHC-P: 1:1000-1:4000
IHC-P: 1:1000-1:4000
Immunogen
Recombinant Mouse GRN protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Granulin (rM Granulin). Granulin specific IgG was purified by mouse Granulin affinity chromatography
.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GRN in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GRN in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GRN in mouse kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GRN in mouse kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-GRN antibody
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
References
Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,458 Da
NCBI Official Full Name
granulins
NCBI Official Synonym Full Names
granulin
NCBI Official Symbol
Grn
NCBI Official Synonym Symbols
Pgrn; epithelin
NCBI Protein Information
granulins
Protein Family
Research Articles on GRN
Similar Products
Product Notes
The GRN (Catalog #AAA8111488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Granulin Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Granulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:1000-1:2000 IHC-P: 1:1000-1:4000. Researchers should empirically determine the suitability of the GRN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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