Rabbit anti-Human, Mouse GPT2 Polyclonal Antibody | anti-GPT2 antibody

GPT2, NT (GPT2, AAT2, ALT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic-alanine transaminase 2, Glutamic-pyruvic transaminase 2) (FITC)

Cat. Num. AAA6304176

• Gene Names: GPT2; ALT2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GPT2; Polyclonal Antibody; NT (GPT2; AAT2; ALT2; Alanine aminotransferase 2; Glutamate pyruvate transaminase 2; Glutamic-alanine transaminase 2; Glutamic-pyruvic transaminase 2) (FITC); Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2; anti-GPT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPT2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GPT2 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 15-44 in the N-terminal region of human GPT2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6006095. This demonstrates that MBS6006095 detected the GPT2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) usingMBS6006095. This demonstrates that MBS6006095 detected the GPT2 protein (arrow).)

Related Product Information for anti-GPT2 antibody

GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.

Product Categories/Family for anti-GPT2 antibody

Antibodies; Enzymes; Aminotransferase

References

1. Glinghammar, B., et al. Int. J. Mol. Med. 23(5):621-631(2009). 2. Fraser, A., et al. Hepatology 46(1):158-165(2007). 3. Ding, Y., et al. World J. Gastroenterol. 12(43):7038-7041(2006). 4. Martin, J., et al. Nature 432(7020):988-994(2004). 5. Yang, R.Z., et al. Genomics 79(3):445-450(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 215599424
• NCBI GeneID: 84706
• NCBI Accession #: NP_001135938.1
• NCBI GenBank Nucleotide #: NM_001142466.1
• UniProt Accession #: Q8TD30; Q8N9E2
• Molecular Weight: 46,983 Da
• NCBI Official Full Name: alanine aminotransferase 2 isoform 2
• NCBI Official Synonym Full Names: glutamic pyruvate transaminase (alanine aminotransferase) 2
• NCBI Official Symbol: GPT2
• NCBI Official Synonym Symbols: ALT2
• NCBI Protein Information: alanine aminotransferase 2; GPT 2; glutamate pyruvate transaminase 2; glutamic--alanine transaminase 2; glutamic--pyruvic transaminase 2; glutamic-pyruvate transaminase 2
• UniProt Protein Name: Alanine aminotransferase 2
• Protein Family: Glycerol-3-phosphate O-acyltransferase
• UniProt Gene Name: GPT2
• UniProt Synonym Gene Names: AAT2; ALT2; ALT2; GPT 2
• UniProt Entry Name: ALAT2_HUMAN

NCBI Description

This gene encodes one of the two pyridoxal enzymes which catalyze a reversible transamination reaction to yield glutamate and pyruvate so these enzymes participate in amino acid metabolism and gluconeogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 2.6.1.2; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: mitochondrial matrix

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine metabolic process; 2-oxoglutarate metabolic process; L-alanine catabolic process

Disease: Mental Retardation, Autosomal Recessive 49