Western Blot (WB)
(Western Blot of 293T/17 whole cell lysate (20ug/lane) using MBS6006785 (1:1000) Secondary IgG, (H+L) (HRP) goat anti-rabbit Predicted band size: 39kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human GPR81 Polyclonal Antibody | anti-GPR81 antibody
GPR81, CT (HCAR1, GPR104, GPR81, HCA1, Hydroxycarboxylic acid receptor 1, G-protein coupled receptor 104, G-protein coupled receptor 81) (MaxLight 405)
Gene Names
HCAR1; HCA1; GPR81; LACR1; FKSG80; GPR104; TAGPCR; TA-GPCR
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
GPR81; Polyclonal Antibody; CT (HCAR1; GPR104; HCA1; Hydroxycarboxylic acid receptor 1; G-protein coupled receptor 104; G-protein coupled receptor 81) (MaxLight 405); HCAR1; G-protein coupled receptor 81; anti-GPR81 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GPR81.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-GPR81 antibody
Western Blot (WB), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 287-316 in the C-terminal region of human GPR81, UniProt Accession #Q9BXC0.
Conjugate
MaxLight405
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot of 293T/17 whole cell lysate (20ug/lane) using MBS6006785 (1:1000) Secondary IgG, (H+L) (HRP) goat anti-rabbit Predicted band size: 39kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot of 293T/17 whole cell lysate (20ug/lane) using MBS6006785 (1:1000) Secondary IgG, (H+L) (HRP) goat anti-rabbit Predicted band size: 39kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GPR81 antibody
G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
Product Categories/Family for anti-GPR81 antibody
References
1. Bailey, S.D., et al. Diabetes Care (2010). 2. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642 (2009). 3. Jeninga, E.H., et al. J. Biol. Chem. 284(39):26385-26393 (2009). 4. Liu, C., et al. J. Biol. Chem. 284(5):2811-2822(2009). 5. Cai, T.Q., et al. Biochem. Biophys. Res. Commun. 377(3):987-991(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,295 Da
NCBI Official Full Name
hydroxycarboxylic acid receptor 1
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 1
NCBI Official Symbol
HCAR1
NCBI Official Synonym Symbols
HCA1; GPR81; LACR1; FKSG80; GPR104; TAGPCR; TA-GPCR
NCBI Protein Information
hydroxycarboxylic acid receptor 1; lactate receptor 1; G protein-coupled receptor 81; G-protein coupled receptor 81; G protein-coupled receptor 104; G-protein coupled receptor 104; hydroxy-carboxylic acid receptor 1; T-cell activation G protein-coupled re
UniProt Protein Name
Hydroxycarboxylic acid receptor 1
UniProt Gene Name
HCAR1
UniProt Synonym Gene Names
GPR104; GPR81; HCA1
UniProt Entry Name
HCAR1_HUMAN
NCBI Description
G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.[supplied by OMIM, Feb 2005]
Uniprot Description
Gpr81: Acts as a receptor for L-lactate and mediates its anti- lipolytic effect through a G(i)-protein-mediated pathway. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; response to estradiol stimulus
Research Articles on GPR81
Similar Products
Product Notes
The GPR81 hcar1 (Catalog #AAA6304035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR81, CT (HCAR1, GPR104, GPR81, HCA1, Hydroxycarboxylic acid receptor 1, G-protein coupled receptor 104, G-protein coupled receptor 81) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR81 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GPR81 hcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR81, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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