Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using GPR175 antibody.)
Rabbit GPR175 Polyclonal Antibody | anti-GPR175 antibody
GPR175 Antibody
Gene Names
TPRA1; GPR175; TPRA40; TMEM227
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR175; Polyclonal Antibody; GPR175 Antibody; FLJ32197; G protein coupled receptor 175; Integral membrane protein GPR175; PP6566; Seven transmembrane domain orphan receptor; TMEM227; Tpra1; TPRA1_HUMAN; TPRA40; Transmembrane domain protein regulated in adipocytes; Transmembrane domain protein regulated in adipocytes 40 kDa; Transmembrane protein 227; Transmembrane protein adipocyte-associated 1; anti-GPR175 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR175 antibody detects endogenous levels of total GPR175
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
373
Applicable Applications for anti-GPR175 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Tissue Specificity
Ubiquitous, with higher levels in heart, placenta and kidney.
Predicted Cross Reactivity
Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken
Similarity
Zebrafish (80%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (87%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using GPR175 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using GPR175 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using GPR175 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using GPR175 antibody.)
Immunofluorescence (IF)
(MBS9606355 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9606355 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-GPR175 antibody
Similarity: Belongs to the UPF0359 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 41 kD
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
transmembrane protein adipocyte-associated 1 isoform 1
NCBI Official Synonym Full Names
transmembrane protein adipocyte associated 1
NCBI Official Symbol
TPRA1
NCBI Official Synonym Symbols
GPR175; TPRA40; TMEM227
NCBI Protein Information
transmembrane protein adipocyte-associated 1
UniProt Protein Name
Transmembrane protein adipocyte-associated 1
UniProt Gene Name
TPRA1
UniProt Synonym Gene Names
GPR175; TMEM227
Research Articles on GPR175
Similar Products
Product Notes
The GPR175 tpra1 (Catalog #AAA9606355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR175 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPR175 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GPR175 tpra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR175, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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