Rabbit GPR161 Polyclonal Antibody | anti-GPR161 antibody

GPR161 polyclonal antibody

Cat. Num. AAA3014851

• Gene Names: MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR161; Polyclonal Antibody; GPR161 polyclonal antibody; Myosin-14; Myosin heavy chain 14; Myosin heavy chain; non-muscle Iic; Non-muscle myosin heavy chain Iic; NMHC II-C; MYH14; KIAA2034; FP17425; anti-GPR161 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Myosin 14 polyclonal antibody detects endogenous levels of Myosin 14 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR161 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human Myosin 14
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Myosin 14 polyclonal antibody at 1:500 dilutionLaneA: H1792 whole cell lysateLaneB: HEK293T whole cell lysateLaneC: HepG2 whole cell lysateLaneD: The liver tissue lysate of MouseLaneE: The liver tissue lysate of Rat)

Related Product Information for anti-GPR161 antibody

Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively. While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions. The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization. Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration.

NCBI and Uniprot Product Information

• NCBI GI #: 116284396
• NCBI GeneID: 79784
• NCBI Accession #: NP_001070654.1
• NCBI GenBank Nucleotide #: NM_001077186.1
• UniProt Accession #: Q7Z406; Q5CZ75; Q6XYE4; Q76B62; Q8WV23; Q96I22; Q9BT27; Q9BW35; Q9H882; B0I1S2; C3TTN4
• Molecular Weight: 227,871 Da
• NCBI Official Full Name: myosin-14 isoform 1
• NCBI Official Synonym Full Names: myosin, heavy chain 14, non-muscle
• NCBI Official Symbol: MYH14
• NCBI Official Synonym Symbols: DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
• NCBI Protein Information: myosin-14; MYH14 variant protein; myosin, heavy polypeptide 14; non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C; myosin heavy chain, non-muscle IIc
• UniProt Protein Name: Myosin-14
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: MYH14
• UniProt Synonym Gene Names: KIAA2034; NMHC II-C
• UniProt Entry Name: MYH14_HUMAN

NCBI Description

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

MYH14: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). High levels of expression are found in small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: growth cone; membrane; axon; myosin II complex; cytoplasm; stress fiber; actomyosin

Molecular Function: actin filament binding; calmodulin binding; microfilament motor activity; actin-dependent ATPase activity; ATP binding

Biological Process: skeletal muscle contraction; regulation of cell shape; axon guidance; skeletal muscle development; sensory perception of sound; actin filament-based movement; metabolic process; actomyosin structure organization and biogenesis; ephrin receptor signaling pathway; generation of action potential

Disease: Peripheral Neuropathy, Myopathy, Hoarseness, And Hearing Loss; Deafness, Autosomal Dominant 4a