Immunofluorescence (IF)
(MBS9606391 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human GPR107 Polyclonal Antibody | anti-GPR107 antibody
GPR107 Antibody
Gene Names
GPR107; GCDRP; LUSTR1; bA138E2.2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR107; Polyclonal Antibody; GPR107 Antibody; bA138E2.2; DKFZp667C222; FLJ16312; FLJ20998; FLJ22591; G protein coupled receptor 107; GCDRP; GPR 107; KIAA1624; Lung seven transmembrane receptor 1; LUSTR1; MGC126118; MGC15440; protein GPR107; RP11 88G17; anti-GPR107 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR107 antibody detects endogenous levels of total GPR107
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
600
Applicable Applications for anti-GPR107 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane; Integral to Membrane.
Predicted Cross Reactivity
Pig, Horse
Similarity
Pig (82%), Horse (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9606391 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9606391 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells using GPR107 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells using GPR107 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-GPR107 antibody
Function: Involved in Golgi-to-ER retrograde transport. Functions as a host factor required for infection by Pseudomonas aeruginosa exotoxin A and Campylobacter jejuni CDT toxins.
Post-translational Modifications: Cleaved by a furin endopeptidase to yield two fragments of 17 and 35 kDa that remain associated via a disulfide bond.
Similarity: Belongs to the LU7TM family.
Post-translational Modifications: Cleaved by a furin endopeptidase to yield two fragments of 17 and 35 kDa that remain associated via a disulfide bond.
Similarity: Belongs to the LU7TM family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 67 kD
Predicted: 67 kDa
Predicted: 67 kDa
NCBI Official Full Name
protein GPR107 isoform 1
NCBI Official Synonym Full Names
G protein-coupled receptor 107
NCBI Official Symbol
GPR107
NCBI Official Synonym Symbols
GCDRP; LUSTR1; bA138E2.2
NCBI Protein Information
protein GPR107
UniProt Protein Name
Protein GPR107
Protein Family
UniProt Gene Name
GPR107
UniProt Synonym Gene Names
KIAA1624; LUSTR1
Uniprot Description
Involved in Golgi-to-ER retrograde transport. Functions as a host factor required for infection by Pseudomonas aeruginosa exotoxin A and Campylobacter jejuni CDT toxins.
Research Articles on GPR107
Similar Products
Product Notes
The GPR107 gpr107 (Catalog #AAA9606391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR107 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR107 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GPR107 gpr107 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR107, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
