GPM6A / Glycoprotein M6A Polyclonal Antibody | anti-GPM6A antibody

Anti-GPM6A / Glycoprotein M6A Antibody (aa232-259) IHC-plus

Cat. Num. AAA2400419

• Gene Names: GPM6A; M6A; GPM6
• Reactivity: Human; Predicted Reactivity: Mouse, Rat, Cow.
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified.
• Synonyms: GPM6A / Glycoprotein M6A; Polyclonal Antibody; Anti-GPM6A / Glycoprotein M6A Antibody (aa232-259) IHC-plus; Rabbit Polyclonal to Human GPM6A / Glycoprotein M6A; Human GPM6A / Glycoprotein M6A; GPM6; Glycoprotein M6A; M6A; Membrane glycoprotein m6-a; anti-GPM6A antibody

• Host: GPM6A/Glycoprotein M6A antibody was produced in Rabbit.
• Reactivity: Human; Predicted Reactivity: Mouse, Rat, Cow.
• Clonality: Polyclonal
• Specificity: This GPM6A antibody is generated from rabbits immunized with KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal regin of human GPM6A.
• Purity/Purification: Protein A purified.
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 278

• Applicable Applications for anti-GPM6A antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: IHC- Paraffin (1:100); Western Blot: (1:1000); Flow Cytometry (1:10-1:50); ; Optimal dilution to be determined by the researcher).
• Immunogen Species: GPM6A / Glycoprotein M6A antibody was raised against Human.
• Epitope: aa232-259
• Target: GPM6A/Glycoprotein M6A
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months.; For long term storage store at -20°C.

Related Product Information for anti-GPM6A antibody

GPM6A Antibody, GPM6 Antibody, Glycoprotein M6A Antibody, M6A Antibody, Membrane glycoprotein m6-a Antibody Description: Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. GPM6A-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. May be involved in neuronal NGF-dependent Ca2+ influx.

NCBI and Uniprot Product Information

• NCBI GI #: 14149619
• NCBI GeneID: 2823
• NCBI Accession #: NP_005268.1
• NCBI GenBank Nucleotide #: NM_005277.4
• NCBI Official Full Name: neuronal membrane glycoprotein M6-a isoform 1
• NCBI Official Synonym Full Names: glycoprotein M6A
• NCBI Official Symbol: GPM6A
• NCBI Official Synonym Symbols: M6A; GPM6
• NCBI Protein Information: neuronal membrane glycoprotein M6-a
• UniProt Protein Name: Neuronal membrane glycoprotein M6-a
• Protein Family: Neuronal membrane glycoprotein
• UniProt Gene Name: GPM6A
• UniProt Synonym Gene Names: M6A; M6a
• UniProt Entry Name: GPM6A_HUMAN

Uniprot Description

GPM6A: Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. GPM6A-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G- protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor. Belongs to the myelin proteolipid protein family.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: cell soma; dendritic spine; filopodium; integral to membrane; neuron projection; plasma membrane

Molecular Function: calcium channel activity; protein binding

Biological Process: neurite morphogenesis; neuron migration; positive regulation of filopodium formation; stem cell differentiation; synaptogenesis