Western Blot (WB)
(The anti-GPI8 Pab is used in Western blot to detect GPI8 in 293 cell lysate.)
Rabbit anti-Human GPI8 Polyclonal Antibody | anti-GPI8 antibody
GPI8, NT (GPI-anchor Transamidase, GPI Transamidase, Phosphatidylinositol-glycan Biosynthesis, Class K Protein, PIG-K, hGPI8, GPI8 Homolog, PIGK) (AP)
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
GPI8; Polyclonal Antibody; NT (GPI-anchor Transamidase; GPI Transamidase; Phosphatidylinositol-glycan Biosynthesis; Class K Protein; PIG-K; hGPI8; GPI8 Homolog; PIGK) (AP); anti-GPI8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GPI8.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-GPI8 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human GPI8 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-GPI8 Pab is used in Western blot to detect GPI8 in 293 cell lysate.)
Western Blot (WB)
(The anti-GPI8 Pab is used in Western blot to detect GPI8 in 293 cell lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-GPI8 antibody
References
1. Ohishi, K., et al., J. Biol. Chem. 278(16):13959-13967 (2003). 2. Vainauskas, S., et al., J. Biol. Chem. 277(34):30535-30542 (2002). 3. Ohishi, K., et al., EMBO J. 20(15):4088-4098 (2001). 4. Meyer, U., et al., Biochemistry 39(12):3461-3471 (2000). 5. Yu, J., et al., Proc. Natl. Acad. Sci. USA 94(23):12580-12585 (1997).
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Product Notes
The GPI8 (Catalog #AAA6478032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPI8, NT (GPI-anchor Transamidase, GPI Transamidase, Phosphatidylinositol-glycan Biosynthesis, Class K Protein, PIG-K, hGPI8, GPI8 Homolog, PIGK) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPI8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GPI8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPI8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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