Rabbit anti-Human, Mouse GPC4 Polyclonal Antibody | anti-GPC4 antibody

GPC4 Antibody

Cat. Num. AAA1492130

• Gene Names: GPC4; K-glypican
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: GPC4; Polyclonal Antibody; GPC4 Antibody; Glypican-4; UNQ474/PRO937; K-glypican; Secreted glypican-4; anti-GPC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 556

• Applicable Applications for anti-GPC4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Glypican-4 protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: GPC4 antibody at 8ug/ml+ Mouse brain tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 63,55 kDaObserved band size: 62 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS1492130 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1492130 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS1492130 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-GPC4 antibody

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 21614525
• NCBI GeneID: 2239
• NCBI Accession #: NP_001439.2
• NCBI GenBank Nucleotide #: NM_001448.2
• UniProt Accession #: O75487; Q6ZMA6; Q96L43; Q9NU08; Q9UJN1; Q9UPD9; B2R6J7; B4E2C0
• Molecular Weight: 54,910 Da
• NCBI Official Full Name: glypican-4
• NCBI Official Synonym Full Names: glypican 4
• NCBI Official Symbol: GPC4
• NCBI Official Synonym Symbols: K-glypican
• NCBI Protein Information: glypican-4
• UniProt Protein Name: Glypican-4
• Protein Family: Glypican
• UniProt Gene Name: GPC4
• UniProt Entry Name: GPC4_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: external side of plasma membrane; extracellular space; Golgi lumen; integral to plasma membrane; lysosomal lumen; nucleus; plasma membrane; proteinaceous extracellular matrix

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: anatomical structure morphogenesis; carbohydrate metabolic process; cell proliferation; chondroitin sulfate metabolic process; fat-soluble vitamin metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; phototransduction, visible light; retinoid metabolic process; vitamin metabolic process

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1