Rabbit anti-Human gp91phox Polyclonal Antibody | anti-gp91phox antibody

gp91phox (PE)

Cat. Num. AAA6478725

• Gene Names: CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: gp91phox; Polyclonal Antibody; gp91phox (PE); anti-gp91phox antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human gp91-phox, Mr 75kD. Depending on the type of lysate, the amount loaded, the gel and the blot conditions, gp91-phox may run as a sharp band, Mr ~75kD, or appear as a smear between 75-91kD.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-gp91phox antibody: Western Blot (WB)
• Application Notes: Not recommended for Immunoprecipitation.; Applications are based on unconjugated antibody.; WB: 1-4ug/ml detects gp91-phox in a HL60 membrane, RIPA cell lysate and human neutrophil membranes. HL60 membrane lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.
• Immunogen: Synthetic peptide corresponding to aa548-560, KQSISNSESGPRG, of human gp91-phox (KLH). Species sequence homology: mouse-100%; bovine-11/13; porcine-10/13.
• Conjugate: PE
• Positive Control: Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: HL60 membrane lysate was probed with MBS610588 (4ug/ml).)

Related Product Information for anti-gp91phox antibody

NADPH Oxidase is comprised of flavocytochrome b558 (made up of p22-phox and gp91-phox), p40-phox, p47-phox and p67-phox. NADPH Oxidase mediates the final steps of electron transfer to oxygen, resulting in the generation of superoxide ion. A growing body of evidence suggests a potential role for oxygen-derived radicals such as superoxide anions and hydrogen peroxide as intracellular signaling molecules that may be involved in cell growth, apoptosis and cellular senescence.

Product Categories/Family for anti-gp91phox antibody

Antibodies; Proteins

References

1. Leusen, J.H., et al., J. Lab. Clin. Med. 128: 461 (1996). 2. Javesghani, D., et al., Am. J. Respir. Crit. Care Med. 165: 412-418 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 115211
• NCBI GeneID: 1536
• NCBI Accession #: P04839.2
• NCBI GenBank Nucleotide #: NM_000397
• UniProt Accession #: P04839; Q2PP16; A8K138
• Molecular Weight: 65,336 Da
• NCBI Official Full Name: Cytochrome b-245 heavy chain
• NCBI Official Synonym Full Names: cytochrome b-245, beta polypeptide
• NCBI Official Symbol: CYBB
• NCBI Official Synonym Symbols: CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• NCBI Protein Information: cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain s
• UniProt Protein Name: Cytochrome b-245 heavy chain
• UniProt Gene Name: CYBB
• UniProt Synonym Gene Names: NOX2; Cytochrome b558 subunit beta
• UniProt Entry Name: CY24B_HUMAN

NCBI Description

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.

Protein type: Oxidoreductase; Mitochondrial; EC 1.-.-.-; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; rough endoplasmic reticulum; cell soma; mitochondrion; integral to plasma membrane; dendrite; plasma membrane; NADPH oxidase complex

Molecular Function: protein binding; FAD binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; voltage-gated ion channel activity

Biological Process: response to drug; respiratory burst; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; ion transport; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process; response to nutrient

Disease: Immunodeficiency 34; Granulomatous Disease, Chronic, X-linked