Rabbit anti-Mouse GOT1L1 Polyclonal Antibody | anti-GOT1L1 antibody

GOT1L1, CT (GOT1L1, Putative aspartate aminotransferase, cytoplasmic 2, Glutamate oxaloacetate transaminase 1-like protein 1, Transaminase A-like protein 1) (Azide free) (HRP)

Cat. Num. AAA6303352

• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GOT1L1; Polyclonal Antibody; CT (GOT1L1; Putative aspartate aminotransferase; cytoplasmic 2; Glutamate oxaloacetate transaminase 1-like protein 1; Transaminase A-like protein 1) (Azide free) (HRP); Transaminase A-like protein 1; anti-GOT1L1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GOT1L1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GOT1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-383 amino acids from the C-terminal region of human GOT1L1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GOT1L1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GOT1L1 antibody detected the GOT1L1 protein (arrow).)

Immunohistochemistry (IHC)

(GOT1L1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GOT1L1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of GOT1L1 Antibody (C-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-GOT1L1 antibody

Antibodies; Enzymes; Aminotransferase

References

Chern, C.J. Cytogenet. Cell Genet. 17(6):338-342(1976).

NCBI and Uniprot Product Information

• NCBI GI #: 117414139
• NCBI GeneID: 137362
• NCBI Accession #: NP_689626.2
• NCBI GenBank Nucleotide #: NM_152413.2
• UniProt Accession #: Q8NHS2; A8MWL4
• Molecular Weight: 47,305 Da
• NCBI Official Full Name: putative aspartate aminotransferase, cytoplasmic 2
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1-like 1
• NCBI Official Symbol: GOT1L1
• NCBI Protein Information: putative aspartate aminotransferase, cytoplasmic 2
• UniProt Protein Name: Putative aspartate aminotransferase, cytoplasmic 2
• Protein Family: Putative aspartate aminotransferase
• UniProt Gene Name: GOT1L1
• UniProt Entry Name: AATC2_HUMAN

Uniprot Description

GOT1L1: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.-; EC 2.6.1.1; Transferase

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: cytoplasm

Molecular Function: transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; biosynthetic process