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Western Blot (WB)
(Western blot analysis of GOT1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse GOT1 Polyclonal Antibody | anti-GOLT1A antibody
GOT1, CT (GOT1, Aspartate aminotransferase, cytoplasmic, Glutamate oxaloacetate transaminase 1, Transaminase A)
Gene Names
GOLT1A; GOT1; CGI-141; YMR292W; HMFN1187; RP11-203F10.3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
GOT1; Polyclonal Antibody; CT (GOT1; Aspartate aminotransferase; cytoplasmic; Glutamate oxaloacetate transaminase 1; Transaminase A); Anti -GOT1; anti-GOLT1A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-GOLT1A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 360-390 from the C-terminal region of human GOT1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of GOT1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GOT1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene.)
Western Blot (WB)
(Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene.)
Immunohistochemistry (IHC)
(GOT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GOT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GOT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GOT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GOT1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GOT1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GOLT1A antibody
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Product Categories/Family for anti-GOLT1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14,887 Da
NCBI Official Full Name
Golgi transport 1 homolog A (S. cerevisiae)
NCBI Official Synonym Full Names
golgi transport 1A
NCBI Official Symbol
GOLT1A
NCBI Official Synonym Symbols
GOT1; CGI-141; YMR292W; HMFN1187; RP11-203F10.3
NCBI Protein Information
vesicle transport protein GOT1A; hGOT1b; putative protein product of HMFN1187
UniProt Protein Name
Vesicle transport protein GOT1A
Protein Family
UniProt Gene Name
GOLT1A
UniProt Synonym Gene Names
GOT1B
UniProt Entry Name
GOT1A_HUMAN
Uniprot Description
Function: May be involved in fusion of ER-derived transport vesicles with the Golgi complex. Ref.4
Subcellular location: Golgi apparatus membrane; Multi-pass membrane protein
By similarity.
Sequence similarities: Belongs to the GOT1 family.
Research Articles on GOLT1A
Similar Products
Product Notes
The GOLT1A golt1a (Catalog #AAA649982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GOT1, CT (GOT1, Aspartate aminotransferase, cytoplasmic, Glutamate oxaloacetate transaminase 1, Transaminase A) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GOT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the GOLT1A golt1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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