Rabbit anti-Human GOLGA5 Polyclonal Antibody | anti-GOLGA5 antibody

GOLGA5, ID (GOLGA5, RETII, RFG5, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein) (AP)

Cat. Num. AAA6303172

• Gene Names: GOLGA5; RFG5; GOLIM5; ret-II
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GOLGA5; Polyclonal Antibody; ID (GOLGA5; RETII; RFG5; Golgin subfamily A member 5; Cell proliferation-inducing gene 31 protein; Golgin-84; Protein Ret-II; RET-fused gene 5 protein) (AP); RET-fused gene 5 protein; anti-GOLGA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GOLGA5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GOLGA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between aa380-408 from the Central region of human GOLGA5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GOLGA5 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GOLGA5 antibody detected the GOLGA5 protein (arrow).)

Flow Cytometry (FC/FACS)

(GOLGA5 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GOLGA5 antibody

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5.

Product Categories/Family for anti-GOLGA5 antibody

Antibodies; Golgi Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Malsam, J., et al. Science 307(5712):1095-1098(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 30260188
• NCBI GeneID: 9950
• NCBI Accession #: NP_005104.2
• NCBI GenBank Nucleotide #: NM_005113.2
• UniProt Accession #: Q8TBA6; O95287; Q03962; Q2TS49; Q9UQQ7; C9JRU1
• Molecular Weight: 78,108 Da
• NCBI Official Full Name: Golgin subfamily A member 5
• NCBI Official Synonym Full Names: golgin A5
• NCBI Official Symbol: GOLGA5
• NCBI Official Synonym Symbols: RFG5; GOLIM5; ret-II
• NCBI Protein Information: golgin subfamily A member 5
• UniProt Protein Name: Golgin subfamily A member 5
• Protein Family: Golgin subfamily
• UniProt Gene Name: GOLGA5
• UniProt Synonym Gene Names: RETII; RFG5
• UniProt Entry Name: GOGA5_HUMAN

NCBI Description

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013]

Uniprot Description

GOLGA5: a member of the golgin family of proteins, whose members localize to the Golgi. Involved in regulating the architecture of the Golgi apparatus. A coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues. Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Two splice variant isoforms have been described.

Protein type: Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: Golgi membrane; Golgi apparatus; Golgi cisterna; cis-Golgi network; membrane; integral to membrane

Molecular Function: protein homodimerization activity; Rab GTPase binding

Biological Process: Golgi organization and biogenesis; Golgi vesicle transport

Disease: Thyroid Carcinoma, Papillary