Goat F(ab')2 Anti-Mouse IgM (u chain specific) Secondary Antibody
Goat F(ab')2 Anti-Mouse IgM, Human ads-BIOT
Reactivity
Mouse
Applications
ELISA, Immunoblot, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Gel filtration chromatography of pepsin digested antibody.
Synonyms
Goat F(ab')2 Anti-Mouse IgM (u chain specific); Polyclonal Secondary Antibody; Goat F(ab')2 Anti-Mouse IgM; Human ads-BIOT; Human ads - Biotin; Goat F(ab')2 Anti-Mouse IgM (u chain specific) secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Purity/Purification
Gel filtration chromatography of pepsin digested antibody.
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for Goat F(ab')2 Anti-Mouse IgM (u chain specific) secondary antibody
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
Application Notes
Research Application(s): Direct immunofluorescent staining of IgM+ mouse B lymphocytes
Indirect immunofluorescent staining in conjunction with mouse IgM monoclonal antibodies
Enzyme-Linked-Immunosorbent-Assay (ELISA)
Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells
ELISA Suggested Dilution: 1:5,000-1:10,000
Indirect immunofluorescent staining in conjunction with mouse IgM monoclonal antibodies
Enzyme-Linked-Immunosorbent-Assay (ELISA)
Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells
ELISA Suggested Dilution: 1:5,000-1:10,000
Source
Pepsin digest of goat anti-mouse IgM..
Cross Absorption
Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Product Categories/Family for Goat F(ab')2 Anti-Mouse IgM (u chain specific) secondary antibody
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Product Notes
The Goat F(ab')2 Anti-Mouse IgM (u chain specific) (Catalog #AAA674377) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat F(ab')2 Anti-Mouse IgM, Human ads-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Goat F(ab')2 Anti-Mouse IgM (u chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot. Research Application(s): Direct immunofluorescent staining of IgM+ mouse B lymphocytes Indirect immunofluorescent staining in conjunction with mouse IgM monoclonal antibodies Enzyme-Linked-Immunosorbent-Assay (ELISA) Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells ELISA Suggested Dilution: 1:5,000-1:10,000. Researchers should empirically determine the suitability of the Goat F(ab')2 Anti-Mouse IgM (u chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat F(ab')2 Anti-Mouse IgM (u chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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