Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) Secondary Antibody
Goat F(ab')2 Anti-Mouse Ig, Human ads-PE
Reactivity
Mouse
Applications
ELISA, Immunoblot, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Gel filtration chromatography of pepsin digested antibody.
Synonyms
Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L); Polyclonal Secondary Antibody; Goat F(ab')2 Anti-Mouse Ig; Human ads-PE; Human ads - Phycoerythrin-Labeled; Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) secondary antibody
Host
Pepsin digest of goat anti-mouse Ig
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
Reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b IgG3, and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Purity/Purification
Gel filtration chromatography of pepsin digested antibody.
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) secondary antibody
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
Application Notes
Working Dilutions:
PE conjugates: <= 0.1 ug/10^6 cells
Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for you specificity need.
PE conjugates: <= 0.1 ug/10^6 cells
Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for you specificity need.
Source
Pepsin digest of goat anti-mouse Ig (H+L).
Cross Absorption
Pooled human sera and purified human paraproteins.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hyrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Product Categories/Family for Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) secondary antibody
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Product Notes
The Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) (Catalog #AAA674346) is a Secondary Antibody produced from Pepsin digest of goat anti-mouse Ig and is intended for research purposes only. The product is available for immediate purchase. The Goat F(ab')2 Anti-Mouse Ig, Human ads-PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot. Working Dilutions: PE conjugates: <= 0.1 ug/10^6 cells Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for you specificity need. Researchers should empirically determine the suitability of the Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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