Goat Anti-Mouse IgG2c (gamma 2c chain specific) Secondary Antibody
Goat Anti-Mouse IgG2c, Human ads-BIOT
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunoblot, Western Blot
Purity
Affinity chromatography on mouse IgG2c covalently linked to agarose.
Synonyms
Goat Anti-Mouse IgG2c (gamma 2c chain specific); Polyclonal Secondary Antibody; Goat Anti-Mouse IgG2c; Human ads-BIOT; Human ads - Biotin; Goat Anti-Mouse IgG2c (gamma 2c chain specific) secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy chain of mouse IgG2c as demonstrated by ELISA. Minimal cross reactivity with human immunoglobulins.
Purity/Purification
Affinity chromatography on mouse IgG2c covalently linked to agarose.
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for Goat Anti-Mouse IgG2c (gamma 2c chain specific) secondary antibody
ELISA; Flow Cytometry; Immunoblot
Application Notes
ELISA Suggested Dilution: 1:5,000-1:20,000
Source
Pooled antisera from goats hyperimmunized with C57BL/6 IgG2c proteins.
Antigen Description
Recently it became evident that inbred mouse strains with the Igh-1b allele do not have the gene for IgG2a and instead express the IgG2c isotype.^1 Commercial anti-IgG2a antibodies cross react inadequately with IgG2c in ELISA and immunoblot and hence, may not be suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD.
Cross Absorption
Mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of (PBS/NaN3) as preservative. Store at 2- 8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of (PBS/NaN3) as preservative. Store at 2- 8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Product Categories/Family for Goat Anti-Mouse IgG2c (gamma 2c chain specific) secondary antibody
References
1. Martin, R.M., J.L. Brady, and A.L. Lew. 1998. J. Immunol. Meth. 212:187.
2. Parsons, M., V.T. Oi, C.M. Huang, and L. A. Herzenberg. 1983. Immunogenetics 18:323.
3. Morgado, M.G., P. Cam, C.Gris-Liebe, P.A. Cazenave, and E.Jouvin-Marche. 1989. EMBO J. 8:3245.
2. Parsons, M., V.T. Oi, C.M. Huang, and L. A. Herzenberg. 1983. Immunogenetics 18:323.
3. Morgado, M.G., P. Cam, C.Gris-Liebe, P.A. Cazenave, and E.Jouvin-Marche. 1989. EMBO J. 8:3245.
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Product Notes
The Goat Anti-Mouse IgG2c (gamma 2c chain specific) (Catalog #AAA674469) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Mouse IgG2c, Human ads-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Goat Anti-Mouse IgG2c (gamma 2c chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunoblot. ELISA Suggested Dilution: 1:5,000-1:20,000. Researchers should empirically determine the suitability of the Goat Anti-Mouse IgG2c (gamma 2c chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Mouse IgG2c (gamma 2c chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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