Goat anti Horse IgM (Fc specific) Secondary Antibody
Goat anti Horse IgM (Fc specific)
Reactivity
The antiserum does not cross-react with any other component of the horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivit
Applications
Immunoprecipitation
Synonyms
Goat anti Horse IgM (Fc specific); Polyclonal Secondary Antibody; Goat anti Horse IgM (Fc specific) secondary antibody
Host
Goat
Reactivity
The antiserum does not cross-react with any other component of the horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivit
Clonality
Polyclonal
Specificity
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal horse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments.
Form/Format
Delipidated, heat inactivated, lyophilized, stable whole antiserum
Applicable Applications for Goat anti Horse IgM (Fc specific) secondary antibody
Immunoprecipitation (IP)
Preservative
No preservative added
Immunogen
Highly purified normal IgM isolated from pooled horse serum. Freund's complete
adjuvant is used in the first step of the immunization procedure.
Absorption
Immunoaffinity adsorbed using in solubilized antigens as required, to eliminate
antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevent the presence of excess adsorbent protein or immune complexes in the antiserum.
Protein Concentrion
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Antibody Titre
Precipitin titre 1 :32 when tested against pooled normal horse serum in agar-block immunodiffusion titration.
Direction of use
In immunoelectrophoresis use 2 uL or equivalent against 120 uL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 uL in a 3 mm diameter center well and 2 uL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivity Note
The antiSerum does not cross-react with any other component of the Horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Packing:Vial with 1 ml lyophilized antiserum.
Lyophilized at +4 degree C = at least 10 years
reconstituted at or below -20 degree C =3-5 years
reconstituted at +4 degree C = 7 days
Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
Packing:Vial with 1 ml lyophilized antiserum.
Lyophilized at +4 degree C = at least 10 years
reconstituted at or below -20 degree C =3-5 years
reconstituted at +4 degree C = 7 days
Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
Related Product Information for Goat anti Horse IgM (Fc specific) secondary antibody
In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma.
Product Categories/Family for Goat anti Horse IgM (Fc specific) secondary antibody
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Product Notes
The Goat anti Horse IgM (Fc specific) (Catalog #AAA571482) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Horse IgM (Fc specific) reacts with The antiserum does not cross-react with any other component of the horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivit and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Horse IgM (Fc specific) can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Goat anti Horse IgM (Fc specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Horse IgM (Fc specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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