Goat Anti-Guinea Pig IgG (H+L) Secondary Antibody
Goat Anti-Guinea Pig IgG (H+L)-HRP
Reactivity
Guinea Pig
Applications
ELISA, Immunoblot, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity chromatography on guinea pig IgG covalently linked to agarose.
Synonyms
Goat Anti-Guinea Pig IgG (H+L); Polyclonal Secondary Antibody; Goat Anti-Guinea Pig IgG (H+L)-HRP; Goat Anti-Guinea Pig IgG(H+L) - HRP; Goat Anti-Guinea Pig IgG (H+L) secondary antibody
Host
Goat
Reactivity
Guinea Pig
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy and light chains of guinea pig IgG as demonstrated by ELISA. May also react with the light chains of other guinea pig immunoglobulins.
Purity/Purification
Affinity chromatography on guinea pig IgG covalently linked to agarose.
Form/Format
Horseradish Peroxidase (HRP) Conjugate
Applicable Applications for Goat Anti-Guinea Pig IgG (H+L) secondary antibody
ELISA; FLISA; Dot-and slot-immunoblotting; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
Application Notes
Working Dilutions:
FLISA:
FITC conjugate 1:200-1:400
ELISA:
AP conjugate 1:1,000-1:4,000
HRP conjugate 1:4,000-1:8,000
BIOT conjugate 1:5,000-1:20,000
Other applications:
Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
WARNING:
Reagents contain sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
FLISA:
FITC conjugate 1:200-1:400
ELISA:
AP conjugate 1:1,000-1:4,000
HRP conjugate 1:4,000-1:8,000
BIOT conjugate 1:5,000-1:20,000
Other applications:
Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
WARNING:
Reagents contain sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Source
Pooled antisera from goats hyperimmunized with guinea pig IgG.
Cross Absorption
NA
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by ELISA and/or Fluorescent ELISA for conformance to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Product Categories/Family for Goat Anti-Guinea Pig IgG (H+L) secondary antibody
Similar Products
Product Notes
The Goat Anti-Guinea Pig IgG (H+L) (Catalog #AAA674842) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Guinea Pig IgG (H+L)-HRP reacts with Guinea Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Goat Anti-Guinea Pig IgG (H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA; FLISA; Dot-and slot-immunoblotting; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot. Working Dilutions: FLISA: FITC conjugate 1:200-1:400 ELISA: AP conjugate 1:1,000-1:4,000 HRP conjugate 1:4,000-1:8,000 BIOT conjugate 1:5,000-1:20,000 Other applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. WARNING: Reagents contain sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. Researchers should empirically determine the suitability of the Goat Anti-Guinea Pig IgG (H+L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Guinea Pig IgG (H+L), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
