Rabbit GNL1 Polyclonal Antibody | anti-GNL1 antibody

GNL1 (Q103) Polyclonal Antibody

Cat. Num. AAA3003622

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GNL1; Polyclonal Antibody; GNL1 (Q103) Polyclonal Antibody; Guanine nucleotide-binding protein-like 1; GTP-binding protein HSR1; HSR1; anti-GNL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GNL1 (Q103) polyclonal antibody detects endogenous levels of GNL1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 607

• Applicable Applications for anti-GNL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 80-120 of Human GNL1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GNL1 (Q103) pAb at 1:1000 dilutionLane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-GNL1 antibody

GNL1 (guanine nucleotide-binding protein-like 1) is a nuclear protein that likely acts as a regulator of the histocompatibility cluster. GNL1 and MMR1, the murine homolog, are localized within or close to the MHC class I region and belong to the MMR1/HSR1 GTP-binding protein family. GTPases, such as GNL1, from the MMR1/HSR1 GTP-binding protein subfamily are circularly rearranged G-motifs that play a critical role in maintaining normal cell growth. Deletion of these genes results in severe growth defects with a marked reduction in mature rRNA species and a concomitant accumulation of the 35S pre-rRNA transcript. Deletion also causes the ribosomal protein RPL25A to fail exportation from the nucleolus. Deletion of any of the G-domain motifs will result in a null phenotype and nuclear/nucleolar localization that lacks the nucleolar export of preribosomes and is accompanied by a distortion of the nucleolar structure. Upon DNA damage GNL1 is phosphorylated by a kinase, possibly Atm or ATR.

NCBI and Uniprot Product Information

• NCBI GI #: 38788319
• NCBI Accession #: NP_005266.2
• NCBI GenBank Nucleotide #: NP_005266.2
• UniProt Accession #: P36915
• Molecular Weight: ~ 69kDa
• NCBI Official Full Name: guanine nucleotide-binding protein-like 1
• UniProt Protein Name: Guanine nucleotide-binding protein-like 1
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNL1
• UniProt Synonym Gene Names: HSR1
• UniProt Entry Name: GNL1_HUMAN

Uniprot Description

GNL1: a putative GTP-binding protein.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; nucleus

Molecular Function: GTPase activity; GTP binding; structural molecule activity

Biological Process: metabolic process; T cell mediated immunity; ribosome biogenesis and assembly; signal transduction; response to DNA damage stimulus