Rabbit GNB3 Polyclonal Antibody | anti-GNB3 antibody

GNB3 (Guanine Nucleotide Binding Protein (G-Protein) beta Polypeptide 3, Transducin beta Chain 3) (AP)

Cat. Num. AAA6418829

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: GNB3; Polyclonal Antibody; GNB3 (Guanine Nucleotide Binding Protein (G-Protein) beta Polypeptide 3; Transducin beta Chain 3) (AP); anti-GNB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes GNB3. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-GNB3 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human GNB3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of mouse liver cells lines, using GNB3 antibody.)

Product Categories/Family for anti-GNB3 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 121011
• NCBI GeneID: 2784
• UniProt Accession #: P16520; Q96B71; Q9BQC0
• Molecular Weight: 340
• NCBI Official Full Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 3
• NCBI Official Symbol: GNB3
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3; transducin beta chain 3; G protein, beta-3 subunit; hypertension associated protein; GTP-binding regulatory protein beta-3
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
• UniProt Gene Name: GNB3
• UniProt Entry Name: GBB3_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Uniprot Description

G-beta 3: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2. Belongs to the WD repeat G protein beta family.

Protein type: G protein; G protein, heterotrimeric beta; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: neuron projection; plasma membrane

Molecular Function: GTPase activity; protein binding; GTPase binding; signal transducer activity; spectrin binding

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of blood pressure; energy reserve metabolic process

Disease: Hypertension, Essential