Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '12206'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
3.58 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '12206' and pd.language_id = 1
Query
Database
1.54 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '12206'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
3.01 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '12206'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '12206' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '12206'
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (243) "Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specific...
$value['app_notes']
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/5
⇄⧉testing_protocols => string (1475) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12206_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12206_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12206_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12206_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12206_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12206_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12206_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (25) "RAT ANTI MOUSE CD169:FITC"
$value['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value->a['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (243) "Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specific...
$value->a['app_notes']
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/5
⇄⧉testing_protocols => string (1475) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value->a['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12206_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12206_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12206_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12206_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12206_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12206_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12206_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (25) "RAT ANTI MOUSE CD169:FITC"
$value->a['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value->a['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value->a['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value->a['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value->a['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value->a['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value->a['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value->d['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (243) "Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specific...
$value->d['app_notes']
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/5
⇄⧉testing_protocols => string (1475) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value->d['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12206_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12206_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12206_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12206_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12206_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12206_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12206_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (25) "RAT ANTI MOUSE CD169:FITC"
$value->d['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value->d['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value->d['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value->d['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value->d['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value->d['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value->d['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
⇄⧉storage_stability => string (350) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value[0]['_source']['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
<b>Immunohistology - Frozen: </b>Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: <b>We recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.</b><br><b>Flow Cytometry: </b>Minimum Dilution: 1/100; Maximum Dilution: 1/1000
⇄⧉testing_protocols => string (1473) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value[0]['_source']['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12204_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12204_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12204_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12204_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12204_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12204_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12204_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (20) "RAT ANTI MOUSE CD169"
$value[0]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[0]['_source']['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value[0]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[0]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value[0]['_source']['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value[0]['_source']['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value[0]['_source']['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
aaa12204 rat monoclonal igg2a 3d6.112 purified igg liquid immunohistology frozen* flow cytometry fc facs immunofluorescence if frozen minimum dilution 1 50 maximum 100 application note mybiosource recommend using fixation with either 2 paraformaldehyde or ethanol for optimal results 1000 testing data staining of mouse lymph node cryosection anti cd169 antibody clone green in a and cd8 yts105.18 red b c is the merged image nuclei counterstained blue dapi low power aaa12204_td immunoperoxidase stained followed by goat mbs235195 as detection reagent aaa12204_td1 high aaa12204_td2 medium aaa12204_td3 peritoneal macrophages cd169:fitc mbs216654 aaa12204_td4 aaa12204_td5 spleen aaa12204_td6 sialoadhesin sialic acid binding ig like lectin siglec1 sn siglec ser sheep erythrocyte receptor 172,769 da cd_antigen sa sn_mouse 226958332 np_035556.3 q62230 nm_011426.3 o55216 q62228 q62229 d3yvz3 d3yvz4 perservative stabilisers 0.09 sodium azide preparation prepared affinity chromatography on protein g from tissue culture supernatant buffer solution phosphate buffered saline target species dilution1 maximum100 either2
⇄⧉storage_stability => string (350) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value[1]['_source']['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (222) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
$value[1]['_source']['app_notes']
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. <br><b>Immunohistology - Frozen: </b>Minimum Dilution: 1/50; Maximum Dilution: 1/250<br><b>Flow Cytometry: </b>Maximum Dilution: 1/500
⇄⧉testing_protocols => string (2903) "Application Data||Immunoperoxidase staining of rat lymph node cryosection wi...
$value[1]['_source']['testing_protocols']
Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power||AAA11973_APP9.jpg!!Application Data||Frozen rat spleen stained with Mouse anti Rat CD169 followed by Goat anti Mouse IgG:HRP (Rat Adosorbed, Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power||AAA11973_APP7.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Medium power||AAA11973_APP6.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power||AAA11973_APP5.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power||AAA11973_APP4.jpg!!Application Data||Published customer image: Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20x magnification).From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.||AAA11973_APP3.jpg!!Application Data||Published customer image: Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.||AAA11973_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Low power||AAA11973_APP.jpg
⇄products_name_oem => string (20) "MOUSE ANTI RAT CD169"
$value[1]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[1]['_source']['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value[1]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[1]['_source']['products_gene_name_syn']
⇄⧉products_description => string (572) "Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surfa...
$value[1]['_source']['products_description']
Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
aaa11973 mouse monoclonal igg2a ed3 purified igg liquid immunohistology frozen flow cytometry fc facs immunofluorescence if immunoprecipitation ip use 10ul of the suggested working dilution to label 106 cells in 100ul minimum 1 50 maximum 250 500 testing data staining rat lymph node cryosection with anti cd169 clone red a and cd4 w3 25 green b c is merged image nuclei counter staind blue using dapi low power aaa11973_td published customer immunostaining spleen serial cut sections were stained mabs towards mcl mhc class ii d human i negative control visualized peroxidase conjugated secondary antibody dab substrate rp pulp pals periarteriolar lymphoid sheath foll follicle mz marginal zone.from lobato pascual saether pc dahle mk gaustad p dissen e et al 2013 macrophage type lectin an activating receptor expressed by phagocytic plos one 8 2 e57406 aaa11973_td1 systemically administered liposomes home primarily splenic zone macrophages a,b healthy rats injected 5 mg kg dii labeled pcls psls cryosections metallophilic cd68 representative experiment shown 20x magnification from bogie jf jorissen w mailleux j nijland pg zelcer n vanmierlo t van horssen stinissen hellings hendriks jj myelin alters inflammatory phenotype ppars acta neuropathol commun aug 43 aaa11973_td2 immunoperoxidase followed horseradish goat mbs235411 as detection reagent medium aaa11973_td3 high aaa11973_td4 aaa11973_td5 aaa11973_td6 igg:hrp adosorbed mbs235196 aaa11973_td7 aaa11973_td8 perservative stabilisers 0.09 sodium azide preparation prepared affinity chromatography on protein buffer solution phosphate buffered saline target species label106 minimum1 maximum250 w325 one8 injected5 aug43
⇄⧉storage_stability => string (325) "Store at -20 degree C only. This product should be stored undiluted. Storage...
$value[2]['_source']['storage_stability']
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
<b>Immunohistology - Frozen: </b>Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: <b>We recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.</b><br><b>Flow Cytometry: </b>Minimum Dilution: 1/100; Maximum Dilution: 1/1000
⇄⧉testing_protocols => string (1475) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value[2]['_source']['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12223_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12223_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12223_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12223_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12223_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12223_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12223_APP.jpg
⇄⧉etc_term1 => string (106) "Preparation||Purified IgG prepared by affinity chromatography on Protein G f...
$value[2]['_source']['etc_term1']
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (34) "RAT ANTI MOUSE CD169:Low Endotoxin"
$value[2]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[2]['_source']['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value[2]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[2]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value[2]['_source']['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value[2]['_source']['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value[2]['_source']['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
aaa12223 rat monoclonal igg2a 3d6.112 low endotoxin purified igg liquid immunohistology frozen* flow cytometry fc facs immunofluorescence if frozen minimum dilution 1 50 maximum 100 application note mybiosource recommend using fixation with either 2 paraformaldehyde or ethanol for optimal results 1000 testing data staining of mouse lymph node cryosection anti cd169 antibody clone mbs216653 green in a and cd8 yts105.18 red b c is the merged image nuclei counterstained blue dapi power aaa12223_td immunoperoxidase stained followed by goat mbs235195 as detection reagent aaa12223_td1 high aaa12223_td2 medium aaa12223_td3 peritoneal macrophages cd169:fitc mbs216654 aaa12223_td4 aaa12223_td5 spleen aaa12223_td6 cd169:low sialoadhesin sialic acid binding ig like lectin siglec1 sn siglec ser sheep erythrocyte receptor 172,769 da cd_antigen sa sn_mouse 226958332 np_035556.3 q62230 nm_011426.3 o55216 q62228 q62229 d3yvz3 d3yvz4 preparation prepared affinity chromatography on protein g from tissue culture supernatant buffer solution phosphate buffered saline target species dilution1 maximum100 either2
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value[3]['_source']['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (243) "Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specific...
$value[3]['_source']['app_notes']
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/5
⇄⧉testing_protocols => string (1475) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value[3]['_source']['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12205_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12205_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12205_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12205_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12205_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12205_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12205_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (25) "RAT ANTI MOUSE CD169:FITC"
$value[3]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[3]['_source']['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value[3]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[3]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value[3]['_source']['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value[3]['_source']['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value[3]['_source']['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
⇄⧉search_terms => string (1145) "aaa12205 rat monoclonal igg2a 3d6.112 fitc purified igg conjugated to fluore...
$value[3]['_source']['search_terms']
aaa12205 rat monoclonal igg2a 3d6.112 fitc purified igg conjugated to fluorescein isothiocyanate isomer 1 liquid flow cytometry fc facs the region of antibodies may bind non specifically cells expressing low affinity receptors this be reduced by using seroblock fcr minimum dilution neat maximum 5 testing data immunofluorescence staining mouse lymph node cryosection with anti cd169 antibody clone mbs216653 green in a and cd8 yts105.18 red b c is merged image nuclei counterstained blue dapi power aaa12205_td immunoperoxidase stained followed goat mbs235195 as detection reagent aaa12205_td1 high aaa12205_td2 medium aaa12205_td3 peritoneal macrophages cd169:fitc aaa12205_td4 aaa12205_td5 frozen spleen aaa12205_td6 sialoadhesin sialic acid binding ig like lectin siglec1 sn siglec ser sheep erythrocyte receptor 172,769 da cd_antigen sa sn_mouse 226958332 np_035556.3 q62230 nm_011426.3 o55216 q62228 q62229 d3yvz3 d3yvz4 perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared chromatography on protein g from tissue culture supernatant buffer solution phosphate buffered saline target species isomer1 maximum5
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value[4]['_source']['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (243) "Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specific...
$value[4]['_source']['app_notes']
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/5
⇄⧉testing_protocols => string (1475) "Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA...
$value[4]['_source']['testing_protocols']
Application Data||Frozen mouse spleen stained with Rat anti Mouse CD169||AAA12206_APP7.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power||AAA12206_APP6.jpg!!Application Data||Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC||AAA12206_APP5.gif!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power||AAA12206_APP4.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power||AAA12206_APP3.jpg!!Application Data||Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power||AAA12206_APP2.jpg!!Application Data||Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power||AAA12206_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄products_name_oem => string (25) "RAT ANTI MOUSE CD169:FITC"
$value[4]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[4]['_source']['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value[4]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[4]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1704) "Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also kno...
$value[4]['_source']['products_description']
Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
⇄products_references => string (3) "N/A"
$value[4]['_source']['products_references']
⇄⧉products_related_diseases => string (211) "Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!...
$value[4]['_source']['products_related_diseases']
Inflammation||19!!Nervous System Diseases||9!!Skin Diseases||7!!Necrosis||7!!Cardiovascular Diseases||6!!Carcinoma||5!!Atherosclerosis||4!!Lung Diseases||4!!Bone Marrow Diseases||4!!Respiratory Tract Diseases||4
⇄⧉search_terms => string (1155) "aaa12206 rat monoclonal igg2a 3d6.112 fitc purified igg conjugated to fluore...
$value[4]['_source']['search_terms']
aaa12206 rat monoclonal igg2a 3d6.112 fitc purified igg conjugated to fluorescein isothiocyanate isomer 1 liquid flow cytometry fc facs the region of antibodies may bind non specifically cells expressing low affinity receptors this be reduced by using seroblock fcr minimum dilution neat maximum 5 testing data immunofluorescence staining mouse lymph node cryosection with anti cd169 antibody clone mbs216653 green in a and cd8 yts105.18 red b c is merged image nuclei counterstained blue dapi power aaa12206_td immunoperoxidase stained followed goat mbs235195 as detection reagent aaa12206_td1 high aaa12206_td2 medium aaa12206_td3 peritoneal macrophages cd169:fitc mbs216654 aaa12206_td4 aaa12206_td5 frozen spleen aaa12206_td6 sialoadhesin sialic acid binding ig like lectin siglec1 sn siglec ser sheep erythrocyte receptor 172,769 da cd_antigen sa sn_mouse 226958332 np_035556.3 q62230 nm_011426.3 o55216 q62228 q62229 d3yvz3 d3yvz4 perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared chromatography on protein g from tissue culture supernatant buffer solution phosphate buffered saline target species isomer1 maximum5
⇄⧉storage_stability => string (350) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value[5]['_source']['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (222) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
$value[5]['_source']['app_notes']
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. <br><b>Immunohistology - Frozen: </b>Minimum Dilution: 1/50; Maximum Dilution: 1/250<br><b>Flow Cytometry: </b>Maximum Dilution: 1/500
⇄⧉testing_protocols => string (2903) "Application Data||Immunoperoxidase staining of rat lymph node cryosection wi...
$value[5]['_source']['testing_protocols']
Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power||AAA11972_APP9.jpg!!Application Data||Frozen rat spleen stained with Mouse anti Rat CD169 followed by Goat anti Mouse IgG:HRP (Rat Adosorbed, Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power||AAA11972_APP7.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Medium power||AAA11972_APP6.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power||AAA11972_APP5.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power||AAA11972_APP4.jpg!!Application Data||Published customer image: Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20x magnification).From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.||AAA11972_APP3.jpg!!Application Data||Published customer image: Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.||AAA11972_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Low power||AAA11972_APP.jpg
⇄products_name_oem => string (20) "MOUSE ANTI RAT CD169"
$value[5]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[5]['_source']['products_name_syn']
⇄products_gene_name => string (5) "CD169"
$value[5]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[5]['_source']['products_gene_name_syn']
⇄⧉products_description => string (572) "Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surfa...
$value[5]['_source']['products_description']
Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
aaa11972 mouse monoclonal igg2a ed3 purified igg liquid immunohistology frozen flow cytometry fc facs immunofluorescence if immunoprecipitation ip use 10ul of the suggested working dilution to label 106 cells in 100ul minimum 1 50 maximum 250 500 testing data staining rat lymph node cryosection with anti cd169 clone red a and cd4 w3 25 green b c is merged image nuclei counter staind blue using dapi low power aaa11972_td published customer immunostaining spleen serial cut sections were stained mabs towards mcl mhc class ii d human i negative control visualized peroxidase conjugated secondary antibody dab substrate rp pulp pals periarteriolar lymphoid sheath foll follicle mz marginal zone.from lobato pascual saether pc dahle mk gaustad p dissen e et al 2013 macrophage type lectin an activating receptor expressed by phagocytic plos one 8 2 e57406 aaa11972_td1 systemically administered liposomes home primarily splenic zone macrophages a,b healthy rats injected 5 mg kg dii labeled pcls psls cryosections metallophilic cd68 representative experiment shown 20x magnification from bogie jf jorissen w mailleux j nijland pg zelcer n vanmierlo t van horssen stinissen hellings hendriks jj myelin alters inflammatory phenotype ppars acta neuropathol commun aug 43 aaa11972_td2 immunoperoxidase followed horseradish goat mbs235411 as detection reagent medium aaa11972_td3 high aaa11972_td4 aaa11972_td5 aaa11972_td6 igg:hrp adosorbed mbs235196 aaa11972_td7 aaa11972_td8 perservative stabilisers 0.09 sodium azide preparation prepared affinity chromatography on protein g buffer solution phosphate buffered saline target species label106 minimum1 maximum250 w325 one8 injected5 aug43
⇄⧉specificity => string (193) "This assay has high sensitivity and excellent specificity for detection of m...
$value[6]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of mouse acetyl-CoA. No significant cross-reactivity or interference between mouse acetyl-CoA and analogues was observed.
⇄purity => string (3) "N/A"
$value[6]['_source']['purity']
⇄form => string (3) "N/A"
$value[6]['_source']['form']
⇄concentration => string (3) "N/A"
$value[6]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[6]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[6]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (755) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[6]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for acetyl-CoA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any acetyl-CoA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for acetyl-CoA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of acetyl-CoA bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (559) "aaa15461 mouse this assay has high sensitivity and excellent specificity for...
$value[6]['_source']['search_terms']
aaa15461 mouse this assay has high sensitivity and excellent specificity for detection of acetyl coa no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15461_td elisa kit partial 12,156 da q9uar5_crypv 4894517 aad32537.1 q9uar5 samples serum plasma cell culture supernates tissue homogenates type quantitative sandwich range 0.78 ng ml 50 < 0.195 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml50
⇄⧉products_description => string (1030) "Background: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that c...
$value[7]['_source']['products_description']
Background: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Acetyl-CoA carboxylase can react with acetyl-CoA and ATP, the products are malonyl-CoA, ADP and inorganic phosphorus. The Acetyl-CoA carboxylase activity will be determined by the increase of inorganic phosphorus. At the end of the reaction period, the dye reagent forms a color with released phosphate, which is measured on a plate reader 635 nm.
⇄products_references => string (3) "N/A"
$value[7]['_source']['products_references']
⇄products_related_diseases => string (3) "N/A"
$value[7]['_source']['products_related_diseases']
⇄products_categories => string (3) "N/A"
$value[7]['_source']['products_categories']
⇄ncbi_full_name => string (3) "N/A"
$value[7]['_source']['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
$value[7]['_source']['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value[7]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value[7]['_source']['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value[7]['_source']['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value[7]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value[7]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (3) "N/A"
$value[7]['_source']['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value[7]['_source']['ncbi_pathways']
⇄sp_protein_name => string (3) "N/A"
$value[7]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (3) "N/A"
$value[7]['_source']['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value[7]['_source']['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value[7]['_source']['sp_gene_name_syn']
⇄sp_entry_name => string (3) "N/A"
$value[7]['_source']['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value[7]['_source']['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value[7]['_source']['sp_interactions']
⇄products_url => string (3) "N/A"
$value[7]['_source']['products_url']
⇄products_viewed => string (1) "0"
$value[7]['_source']['products_viewed']
⇄⧉search_terms => string (238) "aaa27886 typical testing data standard curve for reference only aaa27886_sc ...
$value[7]['_source']['search_terms']
aaa27886 typical testing data standard curve for reference only aaa27886_sc assay kit acetyl coa carboxylase microplate samples tissue extracts cell lysate culture media and other biological fluids detection range 1 umol l 100 range1 l100
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
$value[8]['_source']['etc_term2']
Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (852) "Intended Uses: This sandwich kit is for the accurate quantitative detection ...
$value[8]['_source']['products_description']
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Acetyl-Coa Carboxylase (also known as ACC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.<br><br>Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ACC antibody. ACC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ACC Antibody is added and binds to ACC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ACC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
⇄products_references => string (3) "N/A"
$value[8]['_source']['products_references']
⇄products_related_diseases => string (3) "N/A"
$value[8]['_source']['products_related_diseases']
⇄products_categories => string (3) "N/A"
$value[8]['_source']['products_categories']
⇄ncbi_full_name => string (22) "acetyl Coa carboxylase"
$value[8]['_source']['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
$value[8]['_source']['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value[8]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value[8]['_source']['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value[8]['_source']['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value[8]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value[8]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (9) "16,010 Da"
$value[8]['_source']['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value[8]['_source']['ncbi_pathways']
⇄sp_protein_name => string (57) "Biotin carboxyl carrier protein of acetyl-CoA carboxylase"
$value[8]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (3) "N/A"
$value[8]['_source']['sp_protein_name_syn']
⇄sp_gene_name => string (4) "accB"
$value[8]['_source']['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value[8]['_source']['sp_gene_name_syn']
⇄sp_entry_name => string (3) "N/A"
$value[8]['_source']['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value[8]['_source']['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value[8]['_source']['sp_interactions']
⇄products_url => string (3) "N/A"
$value[8]['_source']['products_url']
⇄products_viewed => string (1) "0"
$value[8]['_source']['products_viewed']
⇄⧉search_terms => string (497) "aaa18940 rat typical testing data standard curve for reference only aaa18940...
$value[8]['_source']['search_terms']
aaa18940 rat typical testing data standard curve for reference only aaa18940_sc elisa kit acetyl coa carboxylase acc 16,010 da biotin carboxyl carrier protein of accb 952352610 krt74355.1 samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 0.05ng ml 40ng sensitivity 0.03ng intra precision within an three known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 x100
⇄⧉etc_term1 => string (213) "Samples||Human Serum, Plasma Or Cell Culture Supernatant And Organizations I...
$value[9]['_source']['etc_term1']
Samples||Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Hmgb-1 Concentration!!Assay Type||Sandwich!!Detection Range||10 ng/ml-0.156 ng/ml!!Sensitivity||0.05 ng/ml.
⇄products_name_oem => string (51) "Human Hydroxymethylglutaryl CoA (HMG-CoA) ELISA Kit"
$value[9]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[9]['_source']['products_name_syn']
⇄products_gene_name => string (7) "HMG-CoA"
$value[9]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[9]['_source']['products_gene_name_syn']
⇄⧉products_description => string (677) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
$value[9]['_source']['products_description']
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HMGB-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄⧉search_terms => string (458) "aaa22646 human no cross reaction with other factors typical testing data sta...
$value[9]['_source']['search_terms']
aaa22646 human no cross reaction with other factors typical testing data standard curve for reference only aaa22646_sc elisa kit hydroxymethylglutaryl coa hmg 51,157 da ao1008_03818 a0a064bhn1_aspoz 635514330 kde86083.1 samples serum plasma or cell culture supernatant and organizations in the natural recombinant hmgb 1 concentration assay type sandwich detection range 10 ng ml 0.156 sensitivity 0.05 intra precision <= 8 inter 12 hmgb1 range10 <=8 inter12
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of M...
$value[10]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of MCOA. No significant cross-reactivity or interference between MCOA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MCOA and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[10]['_source']['purity']
⇄form => string (3) "N/A"
$value[10]['_source']['form']
⇄concentration => string (3) "N/A"
$value[10]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 ng/mL
⇄etc_term2 => string (3) "N/A"
$value[10]['_source']['etc_term2']
⇄products_price => string (6) "0.0000"
$value[10]['_source']['products_price']
⇄products_weight => string (4) "5.00"
$value[10]['_source']['products_weight']
⇄products_status => boolean true
$value[10]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
$value[10]['_source']['products_tax_class_id']
⇄manufacturers_id => string (3) "720"
$value[10]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[10]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[10]['_source']['language_id']
⇄products_name => string (11) "Malonyl CoA"
$value[10]['_source']['products_name']
⇄products_name_oem => string (27) "Human Malonyl CoA ELISA Kit"
$value[10]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[10]['_source']['products_name_syn']
⇄products_gene_name => string (11) "Malonyl CoA"
$value[10]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[10]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1389) "Principle of the Assay: MCOA ELISA kit applies the competitive enzyme immuno...
$value[10]['_source']['products_description']
Principle of the Assay: MCOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MCOA antibody and an MCOA-HRP conjugate. The assay sample and buffer are incubated together with MCOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCOA concentration since MCOA from samples and MCOA-HRP conjugate compete for the anti-MCOA antibody binding site. Since the number of sites is limited, as more sites are occupied by MCOA from the sample, fewer sites are left to bind MCOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCOA concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This MCOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MCOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄sp_protein_name => string (45) "Malonyl CoA-acyl carrier protein transacylase"
$value[10]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (3) "N/A"
$value[10]['_source']['sp_protein_name_syn']
⇄sp_gene_name => string (4) "fabD"
$value[10]['_source']['sp_gene_name']
⇄sp_gene_name_syn => string (10) "SENTW_2059"
$value[10]['_source']['sp_gene_name_syn']
⇄sp_entry_name => string (12) "E9A321_SALET"
$value[10]['_source']['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value[10]['_source']['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value[10]['_source']['sp_interactions']
⇄products_url => string (3) "N/A"
$value[10]['_source']['products_url']
⇄products_viewed => string (1) "0"
$value[10]['_source']['products_viewed']
⇄⧉search_terms => string (626) "aaa17280 human this assay has high sensitivity and excellent specificity for...
$value[10]['_source']['search_terms']
aaa17280 human this assay has high sensitivity and excellent specificity for detection of m coa no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17280_sc elisa kit malonyl 32,405 da acyl carrier protein transacylase fabd sentw_2059 e9a321_salet 409250538 yp_006886348.1 nt_187103.1 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 ng ml competitive1.0
⇄⧉testing_protocols => string (4106) "IF (Immunofluorescence)||AAA30931 staining NIH/3T3 cells by IF/ICC. The samp...
$value[11]['_source']['testing_protocols']
IF (Immunofluorescence)||AAA30931 staining NIH/3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.||AAA30931_IF12.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC11.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC10.jpg!!IHC (Immunohistchemistry)||AAA30931 at 1/200 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC9.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC8.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC7.jpg!!IHC (Immunohistchemistry)||AAA30931 at 1/200 staining human gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC6.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC5.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC4.jpg!!IHC (Immunohistochemistry)||AAA30931 at 1/200 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA30931_IHC3.jpg!!WB (Western Blot)||Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Acetyl-p53 (Lys317) Antibody. The lane on the left is treated with the synthesized peptide.||AAA30931_WB2.jpg
⇄⧉etc_term1 => string (1608) "Immunogen||The antiserum was produced against synthesized peptide derived fr...
$value[11]['_source']['etc_term1']
Immunogen||The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys317.!!Subcellular Location||Cytoplasm; Cytoplasm. Nucleus. Nucleus > PML Body. Endoplasmic Reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.!!Tissue Specificity||Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
Antigen NY-CO-13; BCC7; Cellular tumor antigen p53; FLJ92943; LFS1; Mutant tumor protein 53; p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; Transformation related protein 53; TRP53; Tumor protein 53; Tumor protein p53; Tumor suppressor p53
⇄products_gene_name => string (4) "TP53"
$value[11]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[11]['_source']['products_gene_name_syn']
⇄⧉products_description => string (9920) "Description: Tumor protein p53, a nuclear protein, plays an essential role i...
$value[11]['_source']['products_description']
Description: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy.<br>Function: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).<br>Subunit Structure: Interacts with AXIN1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1 (By similarity). Binds DNA as a homotetramer. Interacts with histone acetyltransferases EP300 and methyltransferases HRMT1L2 and CARM1, and recruits them to promoters. In vitro, the interaction of TP53 with cancer-associated/HPV (E6) viral proteins leads to ubiquitination and degradation of TP53 giving a possible model for cell growth regulation. This complex formation requires an additional factor, E6-AP, which stably associates with TP53 in the presence of E6. Interacts (via C-terminus) with TAF1; when TAF1 is part of the TFIID complex. Interacts with ING4; this interaction may be indirect. Found in a complex with CABLES1 and TP73. Interacts with HIPK1, HIPK2, and TP53INP1. Interacts with WWOX. May interact with HCV core protein. Interacts with USP7 and SYVN1. Interacts with HSP90AB1. Interacts with CHD8; leading to recruit histone H1 and prevent transactivation activity (By similarity). Interacts with ARMC10, BANP, CDKN2AIP, NUAK1, STK11/LKB1, UHRF2 and E4F1. Interacts with YWHAZ; the interaction enhances TP53 transcriptional activity. Phosphorylation of YWHAZ on 'Ser-58' inhibits this interaction. Interacts (via DNA-binding domain) with MAML1 (via N-terminus). Interacts with MKRN1. Interacts with PML (via C-terminus). Interacts with MDM2; leading to ubiquitination and proteasomal degradation of TP53. Directly interacts with FBXO42; leading to ubiquitination and degradation of TP53. Interacts (phosphorylated at Ser-15 by ATM) with the phosphatase PP2A-PPP2R5C holoenzyme; regulates stress-induced TP53-dependent inhibition of cell proliferation. Interacts with PPP2R2A. Interacts with AURKA, DAXX, BRD7 and TRIM24. Interacts (when monomethylated at Lys-382) with L3MBTL1. Isoform 1 interacts with isoform 2 and with isoform 4. Interacts with GRK5. Binds to the CAK complex (CDK7, cyclin H and MAT1) in response to DNA damage. Interacts with CDK5 in neurons. Interacts with AURKB, SETD2, UHRF2 and NOC2L. Interacts (via N-terminus) with PTK2/FAK1; this promotes ubiquitination by MDM2. Interacts with PTK2B/PYK2; this promotes ubiquitination by MDM2. Interacts with PRKCG. Interacts with PPIF; the association implicates preferentially tetrameric TP53, is induced by oxidative stress and is impaired by cyclosporin A (CsA). Interacts with human cytomegalovirus/HHV-5 protein UL123. Interacts with SNAI1; the interaction induces SNAI1 degradation via MDM2-mediated ubiquitination and inhibits SNAI1-induced cell invasion. Interacts with KAT6A. Interacts with UBC9. Interacts with ZNF385B; the interaction is direct. Interacts (via DNA-binding domain) with ZNF385A; the interaction is direct and enhances p53/TP53 transactivation functions on cell-cycle arrest target genes, resulting in growth arrest. Interacts with ANKRD2. Interacts with RFFL and RNF34; involved in p53/TP53 ubiquitination. Interacts with MTA1 and COP1. Interacts with CCAR2 (via N-terminus). Interacts (via N-terminus) with human adenovirus 5 E1B-55K protein; this interaction leads to the inhibition of TP53 function and/or its degradation (PubMed:25772236). Interacts with MORC3 (PubMed:17332504). Interacts (via C-terminus) with POU4F2 isoform 1 (via C-terminus) (PubMed:17145718). Interacts (via oligomerization region) with NOP53; the interaction is direct and may prevent the MDM2-mediated proteasomal degradation of TP53 (PubMed:22522597). Interacts with AFG1L; mediates mitochondrial translocation of TP53 (PubMed:27323408). Interacts with UBD (PubMed:25422469).<br>Post-translational Modifications: Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner. Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was initially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 (PubMed:21317932). It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage. Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A. May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line. Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation (PubMed:10722742, PubMed:12810724, PubMed:15340061, PubMed:17170702, PubMed:19880522). Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:10722742, PubMed:12810724, PubMed:20173098). Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation (PubMed:19536131). Deubiquitinated by USP10, leading to its stabilization (PubMed:20096447). Ubiquitinated by TRIM24, RFFL, RNF34 and RNF125, which leads to proteasomal degradation (PubMed:19556538). Ubiquitination by TOPORS induces degradation (PubMed:19473992). Deubiquitination by USP7, leading to stabilization (PubMed:15053880). Isoform 4 is monoubiquitinated in an MDM2-independent manner (PubMed:15340061). Ubiquitinated by COP1, which leads to proteasomal degradation (PubMed:19837670). Ubiquitination and subsequent proteasomal degradation is negatively regulated by CCAR2 (PubMed:25732823). Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by KMT5A, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Dimethylation at Lys-370 and Lys-382 diminishes p53 ubiquitination, through stabilizing association with the methyl reader PHF20. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation. Sumoylated with SUMO1. Sumoylated at Lys-386 by UBC9.<br>Similarity: The nuclear export signal acts as a transcriptional repression domain. The TADI and TADII motifs (residues 17 to 25 and 48 to 56) correspond both to 9aaTAD motifs which are transactivation domains present in a large number of yeast and animal transcription factors. Belongs to the p53 family.
AMPK Signaling Pathway||198868!!Activation Of BH3-only Proteins Pathway||105658!!Activation Of NOXA And Translocation To Mitochondria Pathway||105660!!Activation Of PUMA And Translocation To Mitochondria Pathway||105661!!Alzheimers Disease Pathway||672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway||83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway||511!!Apoptosis Pathway||198797!!Apoptosis Pathway||83060!!Apoptosis Pathway||470
⇄⧉search_terms => string (2185) "aaa30931 rabbit human mouse rat polyclonal igg affinity purification liquid ...
$value[11]['_source']['search_terms']
aaa30931 rabbit human mouse rat polyclonal igg affinity purification liquid phosphate buffered saline ph 7.4 150mm nacl 0.02 sodium azide and 50 glycerol acetyl p53 lys317 antibody detects endogenous levels of total protein only when acetylated at lysine317 western blot wb immunohistochemisty ihc immunofluorescence if immunocytochemistry icc elisa eia 1:500 1:1000 1:50 1:200 staining mda mb 435 by the sample were fixed with pfa permeabilized in 0.1 triton x 100 then blocked 10 serum for 45 minutes 25 degree c primary was diluted 1 200 incubated hour 37 an alexa fluor 594 conjugated goat anti h+l ab 600 used as secondary aaa30931_if analysis extracts from hela cells treated tsa 400nm 24h using lane on left is synthesized peptide aaa30931_wb2 immunohistochemistry gastric tissue sections p formaldehyde a heat mediated antigen retrieval step citrate buffer performed 1.5 hours 22 hrp aaa30931_ihc3 brain aaa30931_ihc4 testicular aaa30931_ihc5 aaa30931_ihc6 skin aaa30931_ihc7 tb aaa30931_ihc8 uterine aaa30931_ihc9 aaa30931_ihc10 ovarian aaa30931_ihc11 nih 3t3 aaa30931_if12 ny co 13 bcc7 cellular tumor flj92943 lfs1 mutant 53 suppressor p53_human phosphoprotein tp53 transformation related trp53 isoform observed kd predicted 44 kda 120407068 np_000537.3 p04637 nm_000546.5 q15086 q15087 q15088 q16535 q16807 q16808 q16809 q16810 q16811 q16848 q2xn98 immunogen antiserum produced against derived around site subcellular location cytoplasm nucleus > pml body endoplasmic reticulum interaction banp promotes nuclear localization recruited into bodies together chek2 localized both most some forms foci that are different nucleoli but found mainly minor predominantly localizes to expressed 4 translocates following cell stress specificity ubiquitous isoforms wide range normal tissues dependent manner 2 not detected lung prostate muscle fetal spinal cord liver 3 spleen testis 7 uterus skeletal breast 8 colon bone marrow intestine 9 heart salivary gland or conjugation unconjugated epitope ph7.4 and50 mb435 in0.1 x100 blocked10 for45 minutes25 diluted1 hour37 fluor594 ab600 performed1.5 hours22 co13 mutant53 predicted44 expressed4 manner2 liver3 testis7 breast8 intestine9
⇄⧉products_description => string (1104) "Intended Uses: This immunoassay kit allows for the in vitro quantitative det...
$value[12]['_source']['products_description']
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉search_terms => string (430) "aaa13354 pig recombinant and natural human protein wnt 5a typical testing da...
$value[12]['_source']['search_terms']
aaa13354 pig recombinant and natural human protein wnt 5a typical testing data standard curve for reference only aaa13354_sc elisa kit acyl coa desaturase delta 9 fatty acid stearoyl scd 1.14.19.1 38,482 da acod_pig 3023238 o02858.1 o02858 samples serum plasma tissue homogenates cell culture supernates or other biological fluids detection range 0.156 10 ng ml sensitivity <0.062 intra assay precision cv <=6.3 inter <=9.5 delta9
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of P...
$value[13]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of PTAU181. No significant cross-reactivity or interference between PTAU181 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTAU181 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[13]['_source']['purity']
⇄form => string (3) "N/A"
$value[13]['_source']['form']
⇄concentration => string (3) "N/A"
$value[13]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄etc_term2 => string (3) "N/A"
$value[13]['_source']['etc_term2']
⇄products_price => string (6) "0.0000"
$value[13]['_source']['products_price']
⇄products_weight => string (4) "5.00"
$value[13]['_source']['products_weight']
⇄products_status => boolean true
$value[13]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
$value[13]['_source']['products_tax_class_id']
⇄manufacturers_id => string (3) "720"
$value[13]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[13]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[13]['_source']['language_id']
⇄products_name => string (22) "Phosphorylated tau 181"
$value[13]['_source']['products_name']
⇄products_name_oem => string (38) "Human Phosphorylated tau 181 ELISA Kit"
$value[13]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[13]['_source']['products_name_syn']
⇄products_gene_name => string (8) "p Tau181"
$value[13]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[13]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1428) "Principle of the Assay: PTAU181 ELISA kit applies the competitive enzyme imm...
$value[13]['_source']['products_description']
Principle of the Assay: PTAU181 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTAU181 antibody and an PTAU181-HRP conjugate. The assay sample and buffer are incubated together with PTAU181-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTAU181 concentration since PTAU181 from samples and PTAU181-HRP conjugate compete for the anti-PTAU181 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTAU181 from the sample, fewer sites are left to bind PTAU181-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTAU181 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This PTAU181 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PTAU181. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (557) "aaa17100 human this assay has high sensitivity and excellent specificity for...
$value[13]['_source']['search_terms']
aaa17100 human this assay has high sensitivity and excellent specificity for detection of ptau181 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17100_sc elisa kit phosphorylated tau 181 p tau181 cardiovascular samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of P...
$value[14]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of PTAU181. No significant cross-reactivity or interference between PTAU181 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTAU181 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[14]['_source']['purity']
⇄form => string (3) "N/A"
$value[14]['_source']['form']
⇄concentration => string (3) "N/A"
$value[14]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄etc_term2 => string (3) "N/A"
$value[14]['_source']['etc_term2']
⇄products_price => string (6) "0.0000"
$value[14]['_source']['products_price']
⇄products_weight => string (4) "5.00"
$value[14]['_source']['products_weight']
⇄products_status => boolean true
$value[14]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
$value[14]['_source']['products_tax_class_id']
⇄manufacturers_id => string (3) "720"
$value[14]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[14]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[14]['_source']['language_id']
⇄products_name => string (22) "Phosphorylated tau 181"
$value[14]['_source']['products_name']
⇄products_name_oem => string (36) "Rat Phosphorylated tau 181 ELISA Kit"
$value[14]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[14]['_source']['products_name_syn']
⇄products_gene_name => string (8) "p-Tau181"
$value[14]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[14]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1426) "Principle of the Assay: PTAU181 ELISA kit applies the competitive enzyme imm...
$value[14]['_source']['products_description']
Principle of the Assay: PTAU181 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTAU181 antibody and an PTAU181-HRP conjugate. The assay sample and buffer are incubated together with PTAU181-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTAU181 concentration since PTAU181 from samples and PTAU181-HRP conjugate compete for the anti-PTAU181 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTAU181 from the sample, fewer sites are left to bind PTAU181-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTAU181 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This PTAU181 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PTAU181. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (555) "aaa17125 rat this assay has high sensitivity and excellent specificity for d...
$value[14]['_source']['search_terms']
aaa17125 rat this assay has high sensitivity and excellent specificity for detection of ptau181 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17125_sc elisa kit phosphorylated tau 181 p tau181 cardiovascular samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of P...
$value[15]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of PTAU231. No significant cross-reactivity or interference between PTAU231 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTAU231 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[15]['_source']['purity']
⇄form => string (3) "N/A"
$value[15]['_source']['form']
⇄concentration => string (3) "N/A"
$value[15]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄etc_term2 => string (3) "N/A"
$value[15]['_source']['etc_term2']
⇄products_price => string (6) "0.0000"
$value[15]['_source']['products_price']
⇄products_weight => string (4) "5.00"
$value[15]['_source']['products_weight']
⇄products_status => boolean true
$value[15]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
$value[15]['_source']['products_tax_class_id']
⇄manufacturers_id => string (3) "720"
$value[15]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[15]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[15]['_source']['language_id']
⇄products_name => string (22) "Phosphorylated tau 231"
$value[15]['_source']['products_name']
⇄products_name_oem => string (38) "Human Phosphorylated tau 231 ELISA Kit"
$value[15]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[15]['_source']['products_name_syn']
⇄products_gene_name => string (8) "p Tau231"
$value[15]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[15]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1428) "Principle of the Assay: PTAU231 ELISA kit applies the competitive enzyme imm...
$value[15]['_source']['products_description']
Principle of the Assay: PTAU231 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTAU231 antibody and an PTAU231-HRP conjugate. The assay sample and buffer are incubated together with PTAU231-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTAU231 concentration since PTAU231 from samples and PTAU231-HRP conjugate compete for the anti-PTAU231 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTAU231 from the sample, fewer sites are left to bind PTAU231-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTAU231 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This PTAU231 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTAU231. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (555) "aaa16202 human this assay has high sensitivity and excellent specificity for...
$value[15]['_source']['search_terms']
aaa16202 human this assay has high sensitivity and excellent specificity for detection of ptau231 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16202_sc elisa kit phosphorylated tau 231 p tau231 neurological samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of P...
$value[16]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of PTAU181. No significant cross-reactivity or interference between PTAU181 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTAU181 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[16]['_source']['purity']
⇄form => string (3) "N/A"
$value[16]['_source']['form']
⇄concentration => string (3) "N/A"
$value[16]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄etc_term2 => string (3) "N/A"
$value[16]['_source']['etc_term2']
⇄products_price => string (6) "0.0000"
$value[16]['_source']['products_price']
⇄products_weight => string (4) "5.00"
$value[16]['_source']['products_weight']
⇄products_status => boolean true
$value[16]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
$value[16]['_source']['products_tax_class_id']
⇄manufacturers_id => string (3) "720"
$value[16]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[16]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[16]['_source']['language_id']
⇄products_name => string (22) "Phosphorylated tau 181"
$value[16]['_source']['products_name']
⇄products_name_oem => string (38) "Mouse Phosphorylated tau 181 ELISA Kit"
$value[16]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[16]['_source']['products_name_syn']
⇄products_gene_name => string (8) "p-Tau181"
$value[16]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[16]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1428) "Principle of the Assay: PTAU181 ELISA kit applies the competitive enzyme imm...
$value[16]['_source']['products_description']
Principle of the Assay: PTAU181 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTAU181 antibody and an PTAU181-HRP conjugate. The assay sample and buffer are incubated together with PTAU181-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTAU181 concentration since PTAU181 from samples and PTAU181-HRP conjugate compete for the anti-PTAU181 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTAU181 from the sample, fewer sites are left to bind PTAU181-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTAU181 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This PTAU181 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PTAU181. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (557) "aaa17095 mouse this assay has high sensitivity and excellent specificity for...
$value[16]['_source']['search_terms']
aaa17095 mouse this assay has high sensitivity and excellent specificity for detection of ptau181 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17095_sc elisa kit phosphorylated tau 181 p tau181 cardiovascular samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
⇄⧉testing_protocols => string (2005) "IHC (Immunohistchemistry)||AAA31057 at 1/200 staining human colon tissue sec...
$value[17]['_source']['testing_protocols']
IHC (Immunohistchemistry)||AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA31057_IHC6.jpg!!IHC (Immunohistochemistry)||AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA31057_IHC5.jpg!!IHC (Immunohistochemistry)||AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.||AAA31057_IHC4.jpg!!IF (Immunofluorescence)||AAA31057 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.||AAA31057_IF3.jpg!!WB (Western Blot)||Western blot analysis of Acetyl-Histone H3 phosphorylation expression in TSA treated RAW264.7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.||AAA31057_WB2.jpg!!WB (Western Blot)||Western blot analysis of Acetyl-Histone H3 phosphorylation expression in mouse muscle and mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.||AAA31057_WB.jpg
⇄⧉etc_term1 => string (399) "Immunogen||A synthesized peptide derived from human Acetyl-Histone H3 around...
$value[17]['_source']['etc_term1']
Immunogen||A synthesized peptide derived from human Acetyl-Histone H3 around the phosphorylation site of Lys9!!Subcellular Location||Nucleus. Chromosome.!!Tissue Specificity||Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.!!<font color="black">Predicted Cross Reactivity</font>||<b>Bovine</b>!!Similarity||Bovine (100%)
⇄⧉products_description => string (5957) "Description: H3F3A Variant histone H3 which replaces conventional H3 in a wi...
$value[17]['_source']['products_description']
Description: H3F3A Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis.<br>Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.<br>Subunit Structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.<br>Post-translational Modifications: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication. Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis. Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711). It gives a specific tag for epigenetic transcription activation (PubMed:29211711).<br>Similarity: Belongs to the histone H3 family.
Alcoholism Pathway||585563!!Alcoholism Pathway||587116!!Amyloids Pathway||366238!!Disease Pathway||530764!!Factors Involved In Megakaryocyte Development And Platelet Production Pathway||187196!!Gene Expression Pathway||105937!!Hemostasis Pathway||106028!!Meiosis Pathway||477133!!Meiotic Recombination Pathway||205242!!RNA Polymerase I Chain Elongation Pathway||106555
⇄⧉products_description => string (828) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[18]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human pSmad2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (348) "aaa13175 human no cross reaction with other factors typical testing data sta...
$value[18]['_source']['search_terms']
aaa13175 human no cross reaction with other factors typical testing data standard curve for reference only aaa13175_sc elisa kit phosphorylated smad 2 psmad2 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 5000 pg ml 78 sensitivity up to 20 intra precision <= 8 inter 12 smad2 ml78 to20 <=8 inter12
IHC (Immunohistchemistry)||HMG-CoA Reductase/HMGCR Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21345_IHC6.jpg!!IHC (Immunohistochemistry)||HMG-CoA Reductase/HMGCR Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21345_IHC5.jpg!!IHC (Immunohistochemistry)||HMG-CoA Reductase/HMGCR Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21345_IHC4.jpg!!IHC (Immunohistochemistry)||HMG-CoA Reductase/HMGCR Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21345_IHC3.jpg!!WB (Western Blot)||HMG-CoA Reductase/HMGCR Antibody-Western blot analysis of HMGCR expression in HepG2 (A); HeLa (B); mouse spleen (C); rat liver (D) whole cell lysates.||AAA21345_WB2.jpg!!ICC (Immunocytochemistry)||HMG-CoA Reductase/HMGCR Antibody-Immunofluorescent analysis of HMGCR staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.||AAA21345_ICC.jpg
⇄⧉etc_term1 => string (113) "Target||Human HMG-CoA Reductase/HMGCR!!Immunogen||Recombinant full length Hu...
$value[19]['_source']['etc_term1']
Target||Human HMG-CoA Reductase/HMGCR!!Immunogen||Recombinant full length Human HMGCR.!!Conjugation||Unconjugated
⇄⧉products_description => string (224) "HMGCR antibody is an unconjugated rabbit polyclonal antibody to HMGCR (HMG-C...
$value[19]['_source']['products_description']
HMGCR antibody is an unconjugated rabbit polyclonal antibody to HMGCR (HMG-CoA Reductase) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
