Rabbit GNAI1 Polyclonal Antibody | anti-GNAI1 antibody

GNAI1 Antibody

Cat. Num. AAA9630780

• Gene Names: GNAI1; Gi
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GNAI1; Polyclonal Antibody; GNAI1 Antibody; Adenylate cyclase inhibiting G alpha protein; Adenylate cyclase inhibitory protein; Adenylate cyclase-inhibiting G alpha protein; G protein alpha inhibiting 1; G protein alpha inhibiting activity polypeptide 1; Gi; Gi inhibitory G protein; Gi1 protein alpha subunit; GNAI 1; GNAI1_HUMAN; Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1; Guanine nucleotide binding protein alpha inhibiting activity polypeptide 1; Guanine nucleotide binding protein G(i) alpha 1 subunit; Guanine nucleotide-binding protein G(i) subunit alpha-1; OTTHUMP00000161319; OTTHUMP00000208047; anti-GNAI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GNAI1 Antibody detects endogenous levels of GNAI1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GNAI1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GNAI1.
• Conjugation: Unconjugated
• Similarity: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
• Post Translational Modifications: Myristoylation at Gly-2 is required for membrane anchoring before palmitoylation.Palmitoylation at Cys-3 varies with membrane lipid composition.(Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
• Subunit Structure: Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. Part of a spindle orientation complex at least composed of GNAI1, GPSM2 and NUMA1. The alpha chain contains the guanine nucleotide binding site. Identified in complex with the beta subunit GNB1 and the gamma subunit GNG1. Identified in complex with the beta subunit GNB1 and the gamma subunit GNG2. GTP binding causes dissociation of the heterotrimer, liberating the individual subunits so that they can interact with downstream effector proteins. Interacts (GDP-bound form) with GPSM1; this inhibits guanine nucleotide exchange and GTP binding (By similarity). Interacts (GDP-bound form) with GPSM2 (via GoLoco domains); this inhibits guanine nucleotide exchange. Interacts with RGS10; this strongly enhances GTP hydrolysis. Interacts with RGS1 and RGS16; this strongly enhances GTPase activity. Interacts with RGS4. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains). Interacts with RGS3, RGS6, RGS7, RGS8, RGS17, RGS18 and RGS20 (in vitro). Interacts (GDP-bound form) with RIC8A (via C-terminus) (By similarity). Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI1 (By similarity). Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif); the interaction leads to activation of GNAI1. Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif).
• Function: Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis. Required for cortical dynein-dynactin complex recruitment during metaphase.
• Note: Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody (PubMed:17635935). Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-GNAI1 antibody

Nucleus; Cytoplasm; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoskeleton; Microtubule organizing center; Centrosome; Cell cortex; Membrane; Lipid-anchor; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 374081863
• NCBI GeneID: 2770
• NCBI Accession #: NP_001243343.1
• NCBI GenBank Nucleotide #: NM_001256414.1
• UniProt Accession #: P63096; P04898; P11015; P31871; Q5U074; Q8TAN5; Q9UGA4; A8KA88; B4E2V1; C9J3A4
• Molecular Weight: 34,775 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(i) subunit alpha-1 isoform 2
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• NCBI Official Symbol: GNAI1
• NCBI Official Synonym Symbols: Gi
• NCBI Protein Information: guanine nucleotide-binding protein G(i) subunit alpha-1; guanine nucleotide-binding protein G(i) subunit alpha-1; Gi1 protein alpha subunit; adenylate cyclase-inhibiting G alpha protein
• UniProt Protein Name: Guanine nucleotide-binding protein G(i) subunit alpha-1
• UniProt Gene Name: GNAI1
• UniProt Entry Name: GNAI1_HUMAN

NCBI Description

Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

G-alpha i1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Interacts with GPSM1. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains); the interaction occurs in the centrosomes. Interacts (GDP-bound form) with RIC8A (via C-terminus). Interacts with DRD2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z)); G protein

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: centrosome; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; nucleus

Molecular Function: GTPase activity; signal transducer activity; protein binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; response to peptide hormone stimulus; platelet activation; synaptic transmission; cell division; metabolic process; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; cell cycle