Rabbit anti-Human GNA13 Polyclonal Antibody | anti-GNA13 antibody

GNA13, ID (GNA13, Guanine nucleotide-binding protein subunit alpha-13) (PE)

Cat. Num. AAA6302874

• Gene Names: GNA13; G13; MGC46138
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GNA13; Polyclonal Antibody; ID (GNA13; Guanine nucleotide-binding protein subunit alpha-13) (PE); Guanine nucleotide-binding protein subunit alpha-13; anti-GNA13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GNA13 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GNA13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the Central region of human GNA13.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GNA13 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GNA13 antibody detected the GNA13 protein (arrow).)

Related Product Information for anti-GNA13 antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Product Categories/Family for anti-GNA13 antibody

Antibodies; Binding Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Grzelinski, M., et al. Clin. Cancer Res. 16(5):1402-1415(2010). Gong, H., et al. Science 327(5963):340-343(2010). Saito, M., et al. Cell. Signal. 22(1):41-46(2010). Chen, L., et al. J. Biol. Chem. 284(40):27409-27415(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 24111250
• NCBI GeneID: 10672
• NCBI Accession #: NP_006563.2
• NCBI GenBank Nucleotide #: NM_006572.4
• UniProt Accession #: Q14344; Q8TD70; B2R977
• Molecular Weight: 1332.7 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit alpha-13
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha 13
• NCBI Official Symbol: GNA13
• NCBI Official Synonym Symbols: G13; MGC46138
• NCBI Protein Information: guanine nucleotide-binding protein subunit alpha-13; g alpha-13; G-protein subunit alpha-13
• UniProt Protein Name: Guanine nucleotide-binding protein subunit alpha-13
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNA13
• UniProt Synonym Gene Names: G alpha-13; G-protein subunit alpha-13
• UniProt Entry Name: GNA13_HUMAN

Uniprot Description

G-alpha 13: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Activates Rho. Protein kinase A blocks Rho activation by phosphorylation of G-alpha(13). Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Regulates cytoplasmic as well as nuclear signaling events such as activation of the Jun N-terminal kinase signaling module, Na+/H+ exchangers, focal adhesion assemblies, and transcriptional activation of specific primary response genes.

Protein type: G protein, heterotrimeric alpha G(12); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: focal adhesion; membrane; brush border membrane; plasma membrane; melanosome; heterotrimeric G-protein complex; nucleus

Molecular Function: GTPase activity; signal transducer activity; protein binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; type 1 angiotensin receptor binding; D5 dopamine receptor binding

Biological Process: phospholipase D activation; platelet activation; metabolic process; in utero embryonic development; signal transduction; G-protein signaling, adenylate cyclase activating pathway; regulation of cell migration; Rho protein signal transduction; patterning of blood vessels; regulation of cell shape; elevation of cytosolic calcium ion concentration; blood coagulation; cell differentiation; cell motility