Rabbit anti-Human Glyoxalase II/HAGH Polyclonal Antibody | anti-HAGH antibody

Anti-Glyoxalase II/HAGH Antibody, Rabbit Polyclonal

Cat. Num. AAA8106214

• Gene Names: HAGH; GLO2; GLX2; GLXII; HAGH1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Glyoxalase II/HAGH; Polyclonal Antibody; Anti-Glyoxalase II/HAGH Antibody; Rabbit Polyclonal; HAGH/GLO2/Glyoxalase II Antibody; Rabbit PAb; Antigen Affinity Purified; hydroxyacylglutathione hydrolase; Anti-GLO2 Antibody; Anti-GLX2 Antibody; Anti-GLXII Antibody; Anti-HAGH1 Antibody; anti-HAGH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HAGH/GLO2/Glyoxalase II
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HAGH antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human HAGH/GLO2/Glyoxalase II protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human HAGH/GLO2/Glyoxalase II (rh HAGH/GLO2/Glyoxalase II). HAGH/GLO2/Glyoxalase II specific IgG was purified by Human HAGH/GLO2/Glyoxalase II affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human HAGH in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human HAGH in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(HAGH was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-HAGH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HAGH rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)

Western Blot (WB)

(Anti-HAGH rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa)

Related Product Information for anti-HAGH antibody

HAGH (Hydroxyacylglutathione Hydrolase) is a Protein Coding gene. 3 alternative splicing and alternative initiation of human isoforms have been reported. The enzyme encoded by this gene is classified as a thioesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. HAGH belongs to the Metallo-beta-lactamase superfamily. HAGH is widely expressed in the kidney, liver, and other tissues. Diseases associated with HAGH include Hydroxyacyl Glutathione Hydrolase Deficiency. Among its related pathways are Pyruvate metabolism and Citric Acid (TCA) cycle and Metabolism. The human and rodent forms of glyoxalase II (HAGH) can readily be separated by starch gel electrophoretic procedures.

References

Mulley JC, et al. (1987). New regional localisations for HAGH and PGP on human chromosome 16. Hum Genet 74 (4): 423-4.Rulli A, et al. (2001). Expression of glyoxalase I and II in normal and breast cancer tissues. Breast Cancer Res. Treat. 66 (1): 67-72.Cordell PA, et al. (2004). The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II. J Biol Chem. 279 (27): 28653-61.

NCBI and Uniprot Product Information

• NCBI GI #: 94538320
• NCBI GeneID: 3029
• NCBI Accession #: NP_001035517.1
• NCBI GenBank Nucleotide #: NM_001040427.1
• UniProt Accession #: Q16775; A8K290; B4DP33; B4DRA7; E7EN93
• Molecular Weight: 25,455 Da
• NCBI Official Full Name: hydroxyacylglutathione hydrolase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: hydroxyacylglutathione hydrolase
• NCBI Official Symbol: HAGH
• NCBI Official Synonym Symbols: GLO2; GLX2; GLXII; HAGH1
• NCBI Protein Information: hydroxyacylglutathione hydrolase, mitochondrial; glx II; glyoxalase II; hydroxyacylglutathione hydroxylase
• UniProt Protein Name: Hydroxyacylglutathione hydrolase, mitochondrial
• UniProt Gene Name: HAGH
• UniProt Synonym Gene Names: GLO2; HAGH1; Glx II
• UniProt Entry Name: GLO2_HUMAN

NCBI Description

The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.1.2.6; Carbohydrate Metabolism - pyruvate; Hydrolase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial matrix; cytoplasm

Molecular Function: hydroxyacylglutathione hydrolase activity; zinc ion binding

Biological Process: glutathione biosynthetic process

Disease: Hydroxyacyl Glutathione Hydrolase Deficiency