Western Blot (WB)
(Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Glyoxalase I Polyclonal Antibody | anti-GLO1 antibody
Glyoxalase I, NT (GLO1, GLOD1, Glx I, GLYI, Aldoketomutase, Ketone-aldehyde Mutase, Lactoylglutathione Lyase, Methylglyoxalase, S-D-lactoylglutathione Methylglyoxal Lyase)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Western Blot (WB)
(Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of widr cells using GLO1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of widr cells using GLO1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.)
Western Blot (WB)
(Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.)
Western Blot (WB)
(Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.)
Western Blot (WB)
(Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.)
NCBI and Uniprot Product Information
NCBI Description
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]
Uniprot Description
GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 4.4.1.5; Lyase; Carbohydrate Metabolism - pyruvate
Chromosomal Location of Human Ortholog: 6p21.3-p21.1
Cellular Component: cytoplasm
Molecular Function: zinc ion binding; lactoylglutathione lyase activity
Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; methylglyoxal metabolic process; negative regulation of apoptosis
Disease: Autism
Research Articles on GLO1
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Product Notes
The GLO1 glo1 (Catalog #AAA630880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glyoxalase I, NT (GLO1, GLOD1, Glx I, GLYI, Aldoketomutase, Ketone-aldehyde Mutase, Lactoylglutathione Lyase, Methylglyoxalase, S-D-lactoylglutathione Methylglyoxal Lyase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glyoxalase I can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the GLO1 glo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glyoxalase I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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