Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Figure. Western Blot: Sample: Recombinant PYGB, Mouse)

Rabbit anti-Mouse Glycogen Phosphorylase, Brain (PYGB) Polyclonal Antibody | anti-PYGB antibody

Polyclonal Antibody to Glycogen Phosphorylase, Brain (PYGB)

Gene Names
PYGB; GPBB
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Glycogen Phosphorylase; Brain (PYGB); Polyclonal Antibody; Polyclonal Antibody to Glycogen Phosphorylase; GPBB; anti-PYGB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PYGB. It has been selected for its ability to recognize PYGB in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.67mg/ml (varies by lot)
Sequence Length
843
Applicable Applications for anti-PYGB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:330-1300
IHC: 5-20ug/mL; 1:33-130
ICC: 5-20ug/mL; 1:33-130
Conjugation
None
Cross Reactivity
Mouse
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot: Sample: Recombinant PYGB, Mouse)

Western Blot (WB) (Figure. Western Blot: Sample: Recombinant PYGB, Mouse)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glycogen phosphorylase, brain form
NCBI Official Synonym Full Names
glycogen phosphorylase B
NCBI Official Symbol
PYGB
NCBI Official Synonym Symbols
GPBB
NCBI Protein Information
glycogen phosphorylase, brain form
UniProt Protein Name
Glycogen phosphorylase, brain form
Protein Family
UniProt Gene Name
PYGB
UniProt Entry Name
PYGB_HUMAN

NCBI Description

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: EC 2.4.1.1; Phosphorylase; Carbohydrate Metabolism - starch and sucrose; Kinase, other; Transferase

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Research Articles on PYGB

Similar Products

Product Notes

The PYGB pygb (Catalog #AAA2111375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glycogen Phosphorylase, Brain (PYGB) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glycogen Phosphorylase, Brain (PYGB) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:330-1300 IHC: 5-20ug/mL; 1:33-130 ICC: 5-20ug/mL; 1:33-130. Researchers should empirically determine the suitability of the PYGB pygb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycogen Phosphorylase, Brain (PYGB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.