Rabbit anti-Rat Glycine Transporter-2 Polyclonal Antibody | anti-slc6a5 antibody

Glycine Transporter-2 (GLYT2) (Glycerol Free)

Cat. Num. AAA616070

• Gene Names: slc6a5; glyt2; slc6a5
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Glycine Transporter-2; Polyclonal Antibody; Glycine Transporter-2 (GLYT2) (Glycerol Free); Anti -Glycine Transporter-2 (GLYT2) (Glycerol Free); anti-slc6a5 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Specific for rat GLYT2. No homology is seen with either GLYT1 or other transporters.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide. Glycerol free.

• Applicable Applications for anti-slc6a5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:50,000 using 50-100ng control peptide/well.
• Immunogen: Synthetic peptide consisting of 20aa sequence within the cytoplasmic, C-terminal domain of rat GLYT2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-slc6a5 antibody

The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms-GLYT1 and GLYT2. Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative splicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and are expressed from the same gene. Although the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b is significantly different in these two species. The GLYT1c subtype has only been reported in humans. GLYT1a is expressed in CNS and peripheral organs. GLYT1b is localized in the CNS. Both neuron and Glial cells have GLYT1. More recent studies indicate that GLYt1b may not be brain specific. Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.

Product Categories/Family for anti-slc6a5 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 157058891
• NCBI GeneID: 100126617
• UniProt Accession #: A7Y2X0
• Molecular Weight: 87,452 Da
• NCBI Official Full Name: glycine transporter 2
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
• NCBI Official Symbol: slc6a5
• NCBI Official Synonym Symbols: glyt2; slc6a5
• NCBI Protein Information: sodium- and chloride-dependent glycine transporter 2; glyT-2; xGlyT2; solute carrier family 6 member 5
• UniProt Protein Name: Sodium- and chloride-dependent glycine transporter 2
• Protein Family: Sodium- and chloride-dependent glycine transporter
• UniProt Gene Name: slc6a5
• UniProt Synonym Gene Names: glyt2
• UniProt Entry Name: SC6A5_XENLA

Uniprot Description

Function: Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses

By similarity. UniProtKB Q9Y345

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Tissue specificity: First expressed in late neurula stages in the anterior spinal cord, where expression intensifies through the tailbud stages, and by hatching, expression is seen in the hindbrain. During late hatching stages, expression extends along most of the length of the spinal cord, mildly intensifies in the hindbrain, and appears in localized regions of the lateral forebrain and medial midbrain. By the swimming tadpole stage, weak expression appears in the anterior hindbrain, with stronger expression in the posterior, post-mitotic neurons. Ref.1

Sequence similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A5 subfamily. [View classification]