Rabbit Glutathione S-Transferase Polyclonal Antibody | anti-GST antibody

Glutathione S-Transferase (GST)-PE

Cat. Num. AAA6478300

• Applications: Western Blot, ELISA
• Purity: Purified by affinity chromatography.
• Synonyms: Glutathione S-Transferase; Polyclonal Antibody; Glutathione S-Transferase (GST)-PE; anti-GST antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Specific for the ~26kD GST protein from S. japonicum and is useful for detecting GST and GST-fusion proteins transcribed from pGEX and similar vectors. Cross-reactivity with endogenous proteins from E. coli bacteria has not been observed.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GST antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1ug/ml; ELISA (solid phase = antigen): 0.1-1.0ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Purified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pGEX vector.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-GST antibody

pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase. Using this fusion system, a polypeptide sequence is linked to the C-terminus of the 26 kD GST protein from Schistosoma japonicum. The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads. The fusion protein is subsequently eluted from the glutathione agarose with an excess of free glutathione. Antibodies to GST are used to detect pGEX-expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins.

Product Categories/Family for anti-GST antibody

Antibodies; Epitope Tag; GST

References

1. Smith, D.B., and Corcoran, L.M. (1991). Expression and Purification of Glutathione-S-Transferase Fusion Proteins In: Current Protocols in Molecular Biology (Unit 16.7). Green Publishing Assoc. Inc, and John Wiley & Sons Inc. 2. Smith, D.B., Davern, K.M., Board, P.G., et. al.; Mr 26, 000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proc. Natl. Acad. Sci. USA 83:8703-8707 (1986). 3. Smith, D.B. and Johnson K.S. (1988). Single-step purification of polypeptides in Escherichia coli as fusions with glutathione Stransferase. Gene 67:31-40.