Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Glutamine Fructose-6-Phosphate Transaminase 1 Polyclonal Antibody | anti-GFPT1 antibody
Polyclonal Antibody to Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded pancreas tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded pancreas tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008]
Uniprot Description
GFAT: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Homotetramer (Potential). Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.6.1.16; Transferase
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: cytosol; extracellular exosome
Molecular Function: glutamine-fructose-6-phosphate transaminase (isomerizing) activity
Biological Process: circadian regulation of gene expression; energy reserve metabolic process; fructose 6-phosphate metabolic process; glutamine metabolic process; IRE1-mediated unfolded protein response; protein N-linked glycosylation; UDP-N-acetylglucosamine biosynthetic process; UDP-N-acetylglucosamine metabolic process
Disease: Myasthenic Syndrome, Congenital, Associated With Acetylcholine Receptor Deficiency; Myasthenic Syndrome, Congenital, With Tubular Aggregates 1
Research Articles on GFPT1
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Product Notes
The GFPT1 gfpt1 (Catalog #AAA2033717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamine Fructose-6-Phosphate Transaminase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the GFPT1 gfpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamine Fructose-6-Phosphate Transaminase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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