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anti-Mouse, Rat Glutamate Transporter EAAT4 Polyclonal Antibody | anti-SLC1A6 antibody

Anti - Glutamate Transporter EAAT4 (Excitatory Amino Acid Transporter 4)

Gene Names
SLC1A6; EAAT4
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Glutamate Transporter EAAT4; Polyclonal Antibody; Anti - Glutamate Transporter EAAT4 (Excitatory Amino Acid Transporter 4); Glutamate Transporter EAAT4 (Excitatory Amino Acid Transporter 4); anti-SLC1A6 antibody
Ordering
For Research Use Only!
Host
Host: Rabbit
Source: Rat
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The peptide was found unique to EAAT4 without significant homology to other glutamate transporters. It is 100% homologous in mouse and 90% in human EAAT4. Antibody crossreactivity in various species is not established.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
564
Applicable Applications for anti-SLC1A6 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique (2). EAAT4 monomers in freshly made rat brain extracts appeared as fuzzy ~65kD protein (2). High MW bands may be see after oxidation (2).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommeded for ELISA, dot blot, or for antibody blocking experiments (use 5-10ug of control peptide per 1ug of aff pure IgG or 1ul of antiserum) to demonstrate antibody specificity
Immunogen
A 21AA synthetic peptide near the cytoplasmic, carboxy terminus of rat EAAT4 (1) was synthesized, conjugated to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SLC1A6 antibody
Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and purkinje cells, and specifically enriched in cortex, hippocampus, and caudate-putamen and confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina.
Product Categories/Family for anti-SLC1A6 antibody
References
• 1. Lin C et al (1997) gene accession# g3023680; Maeno-Hikichi Y et al (1997) Brain Res. Mol. Brain. Res. 48, 176-180; Fairbanks WA et a (1995) Nature 375, 599-6032. Dehnes Y et al (1998) J Neurosci. 18, 3606-3619; Furuta A et al (1997) J Neurosci. 17, 8363-8375.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
61,466 Da
NCBI Official Full Name
glutamate transporter
NCBI Official Symbol
SLC1A6
NCBI Official Synonym Symbols
EAAT4
NCBI Protein Information
excitatory amino acid transporter 4; glutamate transporter; solute carrier family 1 member 6; sodium-dependent glutamate/aspartate transporter
UniProt Protein Name
Excitatory amino acid transporter 4
UniProt Gene Name
SLC1A6
UniProt Synonym Gene Names
EAAT4
UniProt Entry Name
EAA4_CANFA

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Product Notes

The SLC1A6 slc1a6 (Catalog #AAA617669) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - Glutamate Transporter EAAT4 (Excitatory Amino Acid Transporter 4) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Transporter EAAT4 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique (2). EAAT4 monomers in freshly made rat brain extracts appeared as fuzzy ~65kD protein (2). High MW bands may be see after oxidation (2). ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommeded for ELISA, dot blot, or for antibody blocking experiments (use 5-10ug of control peptide per 1ug of aff pure IgG or 1ul of antiserum) to demonstrate antibody specificity. Researchers should empirically determine the suitability of the SLC1A6 slc1a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate Transporter EAAT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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