Rabbit anti-Bovine Glutamate Dehydrogenase Polyclonal Antibody | anti-GLUD1 antibody

Glutamate Dehydrogenase (GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 1 mitochondrial, MGC132003) (HRP)

Cat. Num. AAA620515

• Gene Names: GLUD1; GDH; GDH1; GLUD; MGC132003
• Reactivity: Bovine
• Applications: ELISA, Western Blot, Immunohistochemistry, Gel Super Shift Assay
• Purity: Highly Purified; Puri ed by delipidation, fractionation and ion exchange chromatography.
• Synonyms: Glutamate Dehydrogenase; Polyclonal Antibody; Glutamate Dehydrogenase (GDH; GLDH; GLUD 1; GLUD; GLUD1; Glutamate dehydrogenase 1; Glutamate dehydrogenase 1 mitochondrial; MGC132003) (HRP); Anti -Glutamate Dehydrogenase (GDH; anti-GLUD1 antibody

• Host: Rabbit
• Reactivity: Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against bovine liver glutamate dehydrogenase, anti-HRP and anti-rabbit serum. Crossreactivity from other tissues and species may occur but have not been determined.
• Purity/Purification: Highly Purified; Puri ed by delipidation, fractionation and ion exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA (IgG and Protease free), 40% glycerol. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-GLUD1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitavle for use in ELISA, Western Blot, Dot Blot, Immunoperoxidase Electron Microscopy and Immunohistochemistry.; Dilution: ELISA: 1:1000-1:4000.
• Immunogen: Bovine liver glutamate dehydrogenase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GLUD1 antibody

Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase GDH1, that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.

Product Categories/Family for anti-GLUD1 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 31799
• NCBI GeneID: 2746
• UniProt Accession #: P00367; Q5TBU3; Q14400; Q53GW3; Q59FQ4; Q9UQV0
• Molecular Weight: 61,398 Da
• NCBI Official Full Name: glutamate dehydrogenase
• NCBI Official Synonym Full Names: glutamate dehydrogenase 1
• NCBI Official Symbol: GLUD1
• NCBI Official Synonym Symbols: GDH; GDH1; GLUD; MGC132003
• NCBI Protein Information: glutamate dehydrogenase 1, mitochondrial; GDH 1; OTTHUMP00000020018; glutamate dehydrogenase (NAD(P)+)
• UniProt Protein Name: Glutamate dehydrogenase 1, mitochondrial
• Protein Family: Glutamate dehydrogenase
• UniProt Gene Name: GLUD1
• UniProt Synonym Gene Names: GLUD
• UniProt Entry Name: DHE3_HUMAN

NCBI Description

This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.

Uniprot Description

GLUD1: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Homohexamer. Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme. Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Protein type: EC 1.4.1.3; Energy Metabolism - nitrogen; Amino Acid Metabolism - arginine and proline; Oxidoreductase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm

Molecular Function: identical protein binding; protein binding; glutamate dehydrogenase activity; GTP binding; glutamate dehydrogenase [NAD(P)+] activity; ADP binding; ATP binding

Biological Process: glutamate catabolic process; substantia nigra development; positive regulation of insulin secretion; glutamine metabolic process; amino acid biosynthetic process; glutamate biosynthetic process

Disease: Hyperinsulinemic Hypoglycemia, Familial, 6