Rabbit anti-Rat GluR1 Polyclonal Antibody | anti-GRIA1 antibody

GluR1, phosphorylated (Ser845) (Glutamate Receptor)

Cat. Num. AAA624418

• Gene Names: GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1; MGC133252
• Reactivity: Rat
• Applications: ELISA, Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by epitope affinity chromatography.
• Synonyms: GluR1; Polyclonal Antibody; phosphorylated (Ser845) (Glutamate Receptor); Anti -GluR1; anti-GRIA1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the ~100kD rat GluR1 protein, phosphorylated at Ser845. Does not react with the non-phosphorylated form of the GluR protein.
• Purity/Purification: Affinity Purified; Purified by epitope affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-GRIA1 antibody: ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA, Western Blot and Dot Blot.; Dilution: Western Blot: 1:1000; Dot Blot: 1:1000
• Immunogen: Synthetic phospho-peptide derived from the rat GluR1 protein.
• Positive Control: Rat brain hippocampal homogenate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-GRIA1 antibody

In the mammalian central nervous system, glutamate and its receptors (GluR) are the major excitatory neurotransmitter and receptor, respectively. Imbalances in glutamatergic function have been implicated in neuronal death following ischemia, hypoglycemia or anoxia, epilepsy, and in neurodegenerative disorders. Based on their activation by different pharmacologic agonists GluR have been classified into two major superfamilies: the ligand-gated ion channel receptors referred to as ionotropic GluR (iGluR), and the G-protein-coupled receptors referred to as metabotropic GluR (mGluR). The iGluR respond to selective agonists and are thus defined as the N-methyl-D-aspartate (NMDA) receptors, the alphaamino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA) responsive receptors (GluR1, 2, 3 and 4) and the kainate responsive receptors (GluR5 and 6). GluR1 is phosphorylated on multiple sites, all are located on the C-terminal of the protein. Ligand induced phosphorylation of GluR1 seems to be site-specific. In one study, using transfected HEK cells and neurons in culture, cyclic AMP-dependent protein kinase specifically phosphorylates Ser845 of GluR11. In addition, protein kinase C specifically phosphorylates Ser831 of GluR12. The phosphorylation pattern of GluR1 by PKA and PKC is consistent with the study of AMPA-Rs in cortical neurins and GluR1 expressed in 293 cells. These results suggested that the function of GluR1 can be regulated by protein phosphorylation.

Product Categories/Family for anti-GRIA1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 167001484
• NCBI GeneID: 2890
• NCBI Accession #: NP_001107655.1
• NCBI GenBank Nucleotide #: NM_001114183.1
• UniProt Accession #: P42261; Q2NKM6; D3DQI4; Q59GL5
• Molecular Weight: 101,506 Da
• NCBI Official Full Name: glutamate receptor 1 isoform 2
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 1
• NCBI Official Symbol: GRIA1
• NCBI Official Synonym Symbols: GLUH1; GLUR1; GLURA; GluA1; HBGR1; MGC133252
• NCBI Protein Information: glutamate receptor 1; AMPA 1; gluR-1; gluR-A; gluR-K1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; AMPA-selective glutamate receptor 1
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: GRIA1
• UniProt Synonym Gene Names: GLUH1; GLUR1
• UniProt Entry Name: GRIA1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, calcium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: postsynaptic membrane; recycling endosome; synaptic vesicle; endoplasmic reticulum membrane; cell surface; cell soma; postsynaptic density; dendrite; dendritic spine; plasma membrane; cell junction

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; glutamate receptor activity; PDZ domain binding

Biological Process: synaptic transmission; synaptic transmission, glutamatergic; long-term memory; ionotropic glutamate receptor signaling pathway; receptor internalization; signal transduction