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Western Blot (WB) (Western Blot Analysis: Rat Cerebellum lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Glutamate Receptor delta1 and 2 (1:5,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Glutamate Receptor Delta 1 and 2 (114kD).)

Rabbit anti-Human, Rat GluR1,2 delta Polyclonal Antibody | anti-GRID1 antibody

GluR1,2 delta (Glutamate Receptor) (FITC)

Gene Names
GRID1; GluD1
Reactivity
Human, Rat
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
GluR1; 2 delta; Polyclonal Antibody; 2 delta (Glutamate Receptor) (FITC); anti-GRID1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the C-Terminus of Glutamate receptor delta 1 and delta 2. Species Crossreactivity: rat and human. Reacts weakly with mouse.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-GRID1 antibody
Western Blot (WB)
Application Notes
WB: 1:5000
Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide to the C-Terminus, conjugated to BSA. Cellular Localization: Cell membrane; Multi-pass membrane protein. Species sequence homology: primates.
Conjugate
FITC
Positive Control
Rat cerebellum tissue lysate and human cerebellum tissue lysate
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: Rat Cerebellum lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Glutamate Receptor delta1 and 2 (1:5,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Glutamate Receptor Delta 1 and 2 (114kD).)

Western Blot (WB) (Western Blot Analysis: Rat Cerebellum lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Glutamate Receptor delta1 and 2 (1:5,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Glutamate Receptor Delta 1 and 2 (114kD).)

Testing Data

(Human Cerebellum lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Glutamate Receptor Delta 1/2. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Glutamate Receptor Delta 1/2 (114kD))

Testing Data (Human Cerebellum lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Glutamate Receptor Delta 1/2. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Glutamate Receptor Delta 1/2 (114kD))
Product Categories/Family for anti-GRID1 antibody
References
1. Matsuda, Keiko, et al. (2006). J. Biol. Chem., 281: 25577-87.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112,131 Da
NCBI Official Full Name
glutamate receptor ionotropic, delta-1
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, delta 1
NCBI Official Symbol
GRID1
NCBI Official Synonym Symbols
GluD1
NCBI Protein Information
glutamate receptor ionotropic, delta-1; gluR delta-1 subunit; glutamate receptor delta-1 subunit
UniProt Protein Name
Glutamate receptor ionotropic, delta-1
UniProt Gene Name
GRID1
UniProt Synonym Gene Names
KIAA1220; GluD1
UniProt Entry Name
GRID1_HUMAN

NCBI Description

This gene encodes a subunit of glutamate receptor channels. These channels mediate most of the fast excitatory synaptic transmission in the central nervous system and play key roles in synaptic plasticity.[provided by RefSeq, Jan 2009]

Uniprot Description

GluR-delta1: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID1 subfamily.

Protein type: Channel, calcium; Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass; Receptor, misc.

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: postsynaptic membrane; dendrite; ionotropic glutamate receptor complex; cell junction

Molecular Function: extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity

Biological Process: synaptic transmission, glutamatergic; ionotropic glutamate receptor signaling pathway; social behavior

Research Articles on GRID1

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Product Notes

The GRID1 grid1 (Catalog #AAA6478058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GluR1,2 delta (Glutamate Receptor) (FITC) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GluR1,2 delta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:5000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GRID1 grid1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GluR1,2 delta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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