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Rabbit GLPF Polyclonal Antibody | anti-GLPF antibody

Rabbit anti-Escherichia coli (strain K12) GLPF Polyclonal Antibody

Gene Names
glpF; ECK3919; JW3898
Reactivity
E Coli (strain K12)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
GLPF; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) GLPF Polyclonal Antibody; GLPF Antibody; Glycerol uptake facilitator protein; Aquaglyceroporin; glpF b3927 JW3898; anti-GLPF antibody
Ordering
Host
Rabbit
Reactivity
E Coli (strain K12)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-GLPF antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Escherichia coli (strain K12) glpF protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,780 Da
NCBI Official Full Name
glycerol facilitator
NCBI Official Symbol
glpF
NCBI Official Synonym Symbols
ECK3919; JW3898
NCBI Protein Information
glycerol facilitator
UniProt Protein Name
Glycerol uptake facilitator protein
UniProt Gene Name
glpF
UniProt Entry Name
GLPF_ECOLI

NCBI Description

The glycerol facilitator, GlpF, allows the facilitated diffusion of glycerol across the inner membrane for utilization inside the cell. [More information is available at EcoCyc: EG10396].

Uniprot Description

Function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.

Cofactor: Binds 2 magnesium ions per subunit.

Subunit structure: Homotetramer

Potential.

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.9.

Domain: Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Miscellaneous: The remarkable property of effective water conductance combined with a strict exclusion of all ions including protons is mediated by two conserved asparagines which force a central water molecule to serve strictly as a hydrogen bond donor to its neighboring water molecules. Assisted by the electrostatic potential generated by two half-membrane spanning loops, this dictates opposite orientations of water molecules in the two halves of the channel, and thus prevents the formation of a "proton wire", while permitting rapid water diffusion.

Sequence similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Research Articles on GLPF

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Product Notes

The GLPF glpf (Catalog #AAA7166256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) GLPF Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's GLPF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the GLPF glpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLPF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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