Rabbit anti-Human GJB3 Polyclonal Antibody | anti-GJB3 antibody

GJB3 (Gap Junction Beta 3 Protein, EKV, Connexin-31, CX31, DFNA2, DFNA2B) (Biotin)

Cat. Num. AAA6418523

• Gene Names: GJB3; EKV; CX31; DFNA2; DFNA2B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: GJB3; Polyclonal Antibody; GJB3 (Gap Junction Beta 3 Protein; EKV; Connexin-31; CX31; DFNA2; DFNA2B) (Biotin); anti-GJB3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJB3.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-GJB3 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human GJB3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of Mouse brain tissue lysate, using GJB3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer tissue using GJB3 antibody..)

Product Categories/Family for anti-GJB3 antibody

Antibodies; Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6014758
• NCBI GeneID: 2707
• UniProt Accession #: O75712; Q2TAZ8; B2R790
• Molecular Weight: 270
• NCBI Official Full Name: Gap junction beta-3 protein
• NCBI Official Synonym Full Names: gap junction protein, beta 3, 31kDa
• NCBI Official Symbol: GJB3
• NCBI Official Synonym Symbols: EKV; CX31; DFNA2; DFNA2B
• NCBI Protein Information: gap junction beta-3 protein; connexin 31; connexin-31
• UniProt Protein Name: Gap junction beta-3 protein
• Protein Family: Gap junction beta-3 protein
• UniProt Gene Name: GJB3
• UniProt Synonym Gene Names: CX31; Cx31
• UniProt Entry Name: CXB3_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

GJB3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB3 are a cause of erythrokeratodermia variabilis (EKV). EKV is a genodermatosis characterized by the appearance of two independent skin lesions: transient figurate erythematous patches and hyperkeratosis that is usually localized but occasionally occurs in its generalized form. Clinical presentation varies significantly within a family and from one family to another. Palmoplantar keratoderma is present in around 50% of cases. Defects in GJB3 are the cause of deafness autosomal dominant type 2B (DFNA2B). DFNA2 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: connexon complex; cytoplasm; gap junction; integral to membrane

Molecular Function: gap junction channel activity

Biological Process: skin development; sensory perception of sound; in utero embryonic development; cell communication; transmembrane transport; placenta development

Disease: Deafness, Autosomal Recessive 1a; Deafness, Autosomal Dominant 2b; Erythrokeratodermia Variabilis Et Progressiva