Rabbit anti-Human, Mouse Girdin Polyclonal Antibody | anti-CCDC88A antibody

Girdin Antibody

Cat. Num. AAA9630326

• Gene Names: CCDC88A; APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Girdin; Polyclonal Antibody; Girdin Antibody; AKT iphosphorylation enhancer; Akt phosphorylation enhancer; APE; Ccdc88a; Coiled coil domain containing 88A; Coiled coil domain containing protein 88A; Coiled-coil domain-containing protein 88A; FLJ10392; G alpha interacting vesicle associated protein; G alpha-interacting vesicle-associated protein; Galpha interacting vesicle associated protein; Girders of actin filament; GIV; GRDN; GRDN_HUMAN; HkRP1; Hook related protein 1; Hook-related protein 1; KIAA1212; anti-CCDC88A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-Girdin (Ser1417) Antibody detects endogenous levels of Phospho-Girdin (Ser1417).
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CCDC88A antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human Phospho-Girdin (Ser1417).
• Conjugation: Unconjugated
• Similarity: The GBA (G-alpha binding and activating) motif mediates binding to the alpha subunits of guanine nucleotide-binding proteins (G proteins). In the presence of tyrosine-autophosphorylated growth factor receptors, the C-terminus folds into an SH2-like region which promotes the stable recruitment of CCDC88A to the growth factor receptors. The SH2-like region is phosphorylated by the growth factor receptors prior to completion of folding. Belongs to the CCDC88 family.
• Post Translational Modifications: Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for delocalization from the cell membrane and for cell migration. Phosphorylated on tyrosine residues which promotes binding to phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1/p85a and enhances PI3K activity. Tyrosine-phosphorylated by both receptor and non-receptor tyrosine kinases in vitro. Tyrosine phosphorylation is required for AKT1-dependent phosphorylation of Ser-1417. Phosphorylation at Ser-1690 by PRKCQ disrupts interaction with GNAI3 and inhibits guanine nucleotide exchange factor activity.
• Subunit Structure: Homodimer. Interacts (via GBA motif) with guanine nucleotide-binding protein G(i) alpha subunits GNAI1, GNAI2 and GNAI3. Also interacts (via GNA motif) with guanine nucleotide-binding protein G(s) alpha subunit GNAS. Interaction with G(i) alpha subunits occurs before interaction with GNAS and is regulated by phosphorylation; phosphorylation at Ser-1675 enhances binding to G(i) alpha subunits while phosphorylation at Ser-1690 abolishes G(i) alpha subunit binding, promoting binding to GNAS. Interacts (via C-terminal SH2-like region) with growth factor receptors EGFR, INSR and KDR/VEGFR2 (via their autophosphorylated cytoplasmic tails). Forms a complex with EGFR and GNAI3 which leads to enhanced EGFR signaling and triggering of cell migration; ligand stimulation is required for recruitment of GNAI3 to the complex. Interacts (tyrosine-phosphorylated form) with phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1/p85a (via SH2 domains); the interaction enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration. Interacts with serine/threonine-protein kinase PRKCQ; the interaction leads to phosphorylation of CCDC88A and inhibition of its guanine nucleotide exchange factor activity. Interacts (via C-terminus) with DISC1; the interaction is direct (By similarity). Interacts with AKT proteins; the interaction is inhibited in the presence of DISC1 (By similarity). Interacts with AKT1/PKB (via C-terminus). The non-phosphorylated form interacts with phosphatidylinositol 4-phosphate [PI(4)P] and weakly with phosphatidylinositol 3-phosphate [PI(3)P]. Interacts with microtubules (By similarity). Interacts with actin.
• Tissue Specificity: Expressed ubiquitously.
• Function: Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration. Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB. Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration. Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation. Essential for the integrity of the actin cytoskeleton. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin.
• Note: Localizes to the cytosol in unstimulated cells while EGF stimulation promotes membrane localization and guanine nucleotide exchange factor activity (PubMed:27864364). Localizes to the cell membrane through interaction with phosphoinositides (PubMed:16139227, PubMed:15882442).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CCDC88A antibody

Cell membrane; Peripheral membrane protein; Cytoplasm; Cytosol; Cytoplasmic vesicle; Cell projection; Lamellipodium; Cytoskeleton; Cilium basal body; Microtubule organizing center; Centrosome; Centriole; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 208022632
• NCBI GeneID: 55704
• NCBI Accession #: NP_001129069.1
• NCBI GenBank Nucleotide #: NM_001135597.1
• UniProt Accession #: Q3V6T2; Q53SF1; Q581G3; Q5HYD0; Q7Z339; Q7Z3C5; A1IGE7; B7ZM78; C9JG83
• Molecular Weight: 216,042 Da
• NCBI Official Full Name: girdin isoform 1
• NCBI Official Synonym Full Names: coiled-coil domain containing 88A
• NCBI Official Symbol: CCDC88A
• NCBI Official Synonym Symbols: APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212
• NCBI Protein Information: girdin; Hook-related protein 1; girders of actin filament; girders of actin filaments; AKT-phosphorylation enhancer; g alpha-interacting vesicle-associated protein
• UniProt Protein Name: Girdin
• Protein Family: Girdin
• UniProt Gene Name: CCDC88A
• UniProt Synonym Gene Names: APE; GRDN; KIAA1212; APE; GIV; HkRP1
• UniProt Entry Name: GRDN_HUMAN

NCBI Description

This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

girdin: a ubiquitously expressed actin binding protein that plays an important role in the formation of stress fibers and lamellipodia. Subsequent to phosphorylation by Akt, Girdin accumulates at the leading edge of migrating cells and appears to be crucial for the integrity of the actin cytoskeleton and for cell migration.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: Golgi apparatus; membrane; lamellipodium; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein kinase B binding; protein homodimerization activity; microtubule binding; actin binding; phosphoinositide binding

Biological Process: lamellipodium biogenesis; cell migration; regulation of protein amino acid phosphorylation; TOR signaling pathway; activation of protein kinase B; regulation of actin cytoskeleton organization and biogenesis; DNA replication; regulation of DNA replication; regulation of cell proliferation