Ghrelin Polyclonal Antibody | anti-GHRL antibody
Anti - Ghrelin
Reactivity
Canine, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Ghrelin; Polyclonal Antibody; Anti - Ghrelin; anti-GHRL antibody
Host
Host: Chicken
Source: Human
Source: Human
Reactivity
Canine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
The 15-aa human sequence is 100% conserved in mouse, canine, rat, 86% in porcine and 66% in bovine ghrelin. Full length ghrelin and des-ghrelin (acylated and non-acylated) are also available for antibody and other studies.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
116
Applicable Applications for anti-GHRL antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. Full length ghrelin is ~3.5kD.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of affinity purified IgG or 1 ul of antiserum) to demonstrate antibody specificity.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. Full length ghrelin is ~3.5kD.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of affinity purified IgG or 1 ul of antiserum) to demonstrate antibody specificity.
Immunogen
A 13 aa peptide, n-octanoylated at Ser3, sequence rom the N-terminus of mature human ghrelin (1) was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-GHRL antibody
Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin (\'ghre\' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor. In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
Product Categories/Family for anti-GHRL antibody
References
• 1. Kojima M et al (1999) Nature 402, 656; Hosoda H et al (2000) J. Biol. Chem. 275, 2199; Nakazato M et al (2001) Nature 409, 194; Tschop et al (2000) Nature 407; 908.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
appetite-regulating hormone
NCBI Official Symbol
GHRL
NCBI Protein Information
appetite-regulating hormone; preproghrelin; ghrelin/obestatin preprohormone
Protein Family
Similar Products
Product Notes
The GHRL (Catalog #AAA617462) is an Antibody produced from Host: Chicken Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Ghrelin reacts with Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ghrelin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. Full length ghrelin is ~3.5kD. ELISA: ~1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of affinity purified IgG or 1 ul of antiserum) to demonstrate antibody specificity. Researchers should empirically determine the suitability of the GHRL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ghrelin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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