Immunofluorescence (IF)
(MBS9605548 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human GHITM Polyclonal Antibody | anti-GHITM antibody
GHITM Antibody
Gene Names
GHITM; DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GHITM; Polyclonal Antibody; GHITM Antibody; Dermal papilla derived protein 2; Dermal papilla-derived protein 2; DERP2; Ghitm; GHITM_HUMAN; Growth hormone inducible transmembrane protein; Growth hormone-inducible transmembrane protein; HSPC282; MICS1; Mitochondrial morphology and cristae structure 1; My021; PTD010; TMBIM5; Transmembrane BAX inhibitor motif containing 5; Transmembrane BAX inhibitor motif containing protein 5; Transmembrane BAX inhibitor motif-containing protein 5; anti-GHITM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GHITM antibody detects endogenous levels of total GHITM
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
345
Applicable Applications for anti-GHITM antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605548 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605548 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using GHITM antibody.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using GHITM antibody.)
Related Product Information for anti-GHITM antibody
Function: Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c.
Similarity: Belongs to the BI1 family.
Similarity: Belongs to the BI1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 37 kD
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
growth hormone-inducible transmembrane protein
NCBI Official Synonym Full Names
growth hormone inducible transmembrane protein
NCBI Official Symbol
GHITM
NCBI Official Synonym Symbols
DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282
NCBI Protein Information
growth hormone-inducible transmembrane protein
UniProt Protein Name
Growth hormone-inducible transmembrane protein
Protein Family
UniProt Gene Name
GHITM
UniProt Synonym Gene Names
DERP2; MICS1; TMBIM5; MICS1
Uniprot Description
Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c.
Research Articles on GHITM
Similar Products
Product Notes
The GHITM ghitm (Catalog #AAA9605548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GHITM Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GHITM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GHITM ghitm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GHITM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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