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Western Blot (WB) (Western blot analysis of GGTLA1 antibody (N-term) in Y79 cell line lysates (35ug/lane). GGTLA1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse GGTLA1 Polyclonal Antibody | anti-GGTLA1 antibody

GGTLA1, NT (Gamma-glutamyltransferase 5, Gamma-glutamyltranspeptidase 5, GGT 5, GGT5, Gamma-glutamyltransferase-like Activity 1, Gamma-glutamyl Transpeptidase-related Enzyme, GGT-rel) (AP)

Gene Names
GGT5; GGL; GGT 5; GGTLA1; GGT-REL
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
GGTLA1; Polyclonal Antibody; NT (Gamma-glutamyltransferase 5; Gamma-glutamyltranspeptidase 5; GGT 5; GGT5; Gamma-glutamyltransferase-like Activity 1; Gamma-glutamyl Transpeptidase-related Enzyme; GGT-rel) (AP); EC=2.3.2.2; Gamma-glutamyltransferase 5 Heavy Chain; Gamma-glutamyltransferase 5 Light Chain; anti-GGTLA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GGTLA1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Sequence Length
586
Applicable Applications for anti-GGTLA1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human GGTLA1 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GGTLA1 antibody (N-term) in Y79 cell line lysates (35ug/lane). GGTLA1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GGTLA1 antibody (N-term) in Y79 cell line lysates (35ug/lane). GGTLA1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GGTLA1 antibody (N-term) in mouse heart tissue lysates (35ug/lane). GGTLA1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GGTLA1 antibody (N-term) in mouse heart tissue lysates (35ug/lane). GGTLA1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain reacted with GGTLA1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain reacted with GGTLA1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GGTLA1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (GGTLA1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-GGTLA1 antibody
References
1. Heisterkamp, N., Hum. Genet. 123(4), 321-332 (2008). 2. Enoiu, M., Free Radic. Biol. Med. 29(9), 825-833 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62.261
NCBI Official Full Name
glutathione hydrolase 5 proenzyme isoform 2
NCBI Official Synonym Full Names
gamma-glutamyltransferase 5
NCBI Official Symbol
GGT5
NCBI Official Synonym Symbols
GGL; GGT 5; GGTLA1; GGT-REL
NCBI Protein Information
glutathione hydrolase 5 proenzyme
UniProt Protein Name
Gamma-glutamyltransferase 5
UniProt Gene Name
GGT5
UniProt Synonym Gene Names
GGTLA1; GGT 5; GGT-rel

NCBI Description

This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).

Research Articles on GGTLA1

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Product Notes

The GGTLA1 ggt5 (Catalog #AAA6477757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GGTLA1, NT (Gamma-glutamyltransferase 5, Gamma-glutamyltranspeptidase 5, GGT 5, GGT5, Gamma-glutamyltransferase-like Activity 1, Gamma-glutamyl Transpeptidase-related Enzyme, GGT-rel) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GGTLA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GGTLA1 ggt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGTLA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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