Rabbit GGT1 Polyclonal Antibody | anti-GGT1 antibody

GGT1 Polyclonal Antibody

Cat. Num. AAA3006975

• Gene Names: GGT1; GGT; GTG; GGTD; CD224; GGT 1; D22S672; D22S732
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GGT1; Polyclonal Antibody; GGT1 Polyclonal Antibody; Cathepsin L1; Cathepsin L; Major excreted protein; MEP; CTSL; CTSL1; anti-GGT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GGT1 polyclonal antibody detects endogenous levels of GGT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 569

• Applicable Applications for anti-GGT1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human GGT1
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GGT1 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate)

Related Product Information for anti-GGT1 antibody

GGT (gamma-glutamyltranspeptidase) acts as a glutathionase and catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. This enzyme is located on the outer surface of the cell membrane and is widely distributed in mammalian tissues involved in absorption and secretion. In humans, hepatic GGT activity is elevated in some liver diseases. GGT1 is released into the bloodstream after liver damage, and an elevated level of the enzyme may be a useful early sign of hepatocellular carcinoma. GGT5 converts leukotriene C4 to leukotriene D4; it does not, however, convert synthetic substrates that are commonly used to assay GGT. In human serum and in human tissues, there is a marked heterogeneity in GGT, but this heterogeneity can be attributed to different glycosylation of the same peptide rather than to the products of different genes.

NCBI and Uniprot Product Information

• NCBI GI #: 47678519
• NCBI GeneID: 2678
• UniProt Accession #: P19440
• Molecular Weight: ~ 61kDa
• NCBI Official Full Name: GGT1
• NCBI Official Synonym Full Names: gamma-glutamyltransferase 1
• NCBI Official Symbol: GGT1
• NCBI Official Synonym Symbols: GGT; GTG; GGTD; CD224; GGT 1; D22S672; D22S732
• NCBI Protein Information: glutathione hydrolase 1 proenzyme
• UniProt Protein Name: Gamma-glutamyltranspeptidase 1
• Protein Family: Gamma-glutamyltranspeptidase
• UniProt Gene Name: GGT1
• UniProt Synonym Gene Names: GGT; GGT 1
• UniProt Entry Name: GGT1_HUMAN

NCBI Description

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

Uniprot Description

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Other Amino Acids Metabolism - glutathione; EC 3.4.19.13; Membrane protein, integral; EC 3.4.19.14; Lipid Metabolism - arachidonic acid; Transferase; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - taurine and hypotaurine; EC 2.3.2.2; Other Amino Acids Metabolism - cyanoamino acid

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane

Molecular Function: protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; spermatogenesis; zymogen activation

Disease: Glutathionuria