Rabbit anti-Human, Mouse GGT1 Polyclonal Antibody | anti-GGT1 antibody

GGT1, NT (GGT1, GGT, Gamma-glutamyltranspeptidase 1, Gamma-glutamyltransferase 1, Glutathione hydrolase 1, Leukotriene-C4 hydrolase, CD224, Gamma-glutamyltranspeptidase 1 heavy chain, Gamma-glutamyltranspeptidase 1 light chain) (Azide free) (HRP)

Cat. Num. AAA6302142

• Gene Names: GGT1; GGT; GTG; GGTD; CD224; GGT 1; D22S672; D22S732
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GGT1; Polyclonal Antibody; NT (GGT1; GGT; Gamma-glutamyltranspeptidase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1; Leukotriene-C4 hydrolase; CD224; Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain) (Azide free) (HRP); Gamma-glutamyltranspeptidase 1 light chain; anti-GGT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 569

• Applicable Applications for anti-GGT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GGT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of human GGT1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GGT1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the GGT1 antibody detected the GGT1 protein (arrow).)

Western Blot (WB)

(GGT1 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GGT1 antibody detected the GGT1 protein (arrow).)

Related Product Information for anti-GGT1 antibody

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Categories/Family for anti-GGT1 antibody

Antibodies; CD Markers

References

Speliotes, E.K., et al. Hepatology 52(3):904-912(2010). Ikeda, M., et al. Scand. J. Clin. Lab. Invest. 70(3):171-179(2010). Fujita, M., et al. Exp. Biol. Med. (Maywood) 235(3):335-341(2010). Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010). Diergaarde, B., et al. Pancreatology 10 (2-3), 194-200 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 73915092
• NCBI GeneID: 2678
• NCBI Accession #: NP_038347.2
• NCBI GenBank Nucleotide #: NP_038347.2
• UniProt Accession #: P19440
• NCBI Official Full Name: glutathione hydrolase 1 proenzyme
• NCBI Official Synonym Full Names: gamma-glutamyltransferase 1
• NCBI Official Symbol: GGT1
• NCBI Official Synonym Symbols: GGT; GTG; GGTD; CD224; GGT 1; D22S672; D22S732
• NCBI Protein Information: glutathione hydrolase 1 proenzyme
• UniProt Protein Name: Gamma-glutamyltranspeptidase 1
• Protein Family: Gamma-glutamyltranspeptidase
• UniProt Gene Name: GGT1
• UniProt Synonym Gene Names: GGT; GGT 1
• UniProt Entry Name: GGT1_HUMAN

NCBI Description

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

Uniprot Description

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Other Amino Acids Metabolism - glutathione; EC 3.4.19.13; Membrane protein, integral; EC 3.4.19.14; Lipid Metabolism - arachidonic acid; Transferase; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - taurine and hypotaurine; EC 2.3.2.2; Other Amino Acids Metabolism - cyanoamino acid

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane

Molecular Function: protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; spermatogenesis; zymogen activation

Disease: Glutathionuria