Goat GGA3 Polyclonal Antibody | anti-GGA3 antibody

Goat anti-GGA3 Antibody

Cat. Num. AAA420437

• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GGA3; Polyclonal Antibody; Goat anti-GGA3 Antibody; golgi associated; gamma adaptin ear containing; ARF binding protein 3; HGNC: 17079; KIAA0154; ADP-ribosylation factor binding protein 3; Golgi-localized; gamma ear-containing; ARF-binding protein 3; GGA3 antibody; ARF binding protein 3 antibody; HGNC: 17079 antibody; KIAA0154 antibody; ADP-ribosylation factor binding protein 3 antibody; ARF-binding protein 3 antibody; anti-GGA3 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EQLSTEVGEVDQ
• Sequence Length: 723

• Applicable Applications for anti-GGA3 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Preliminary experiments gave an approx 65kDa band in Mouse and Rat Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78. 0kDa according to NP_766636.2 and 70.2kDa according to NP_001238996.1. The 65kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-EQLSTEVGEVDQ, from the C Terminus of the protein sequence according to NP_619525.1; NP_054720.1.
• Epitope: C Terminus
• Note: This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 20336267
• NCBI GeneID: 23163
• NCBI Accession #: NP_619525.1; NP_054720.1
• NCBI GenBank Nucleotide #: NM_138619.3
• Molecular Weight: 70,148 Da
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA3 isoform long
• NCBI Official Synonym Full Names: golgi associated, gamma adaptin ear containing, ARF binding protein 3
• NCBI Official Symbol: GGA3
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA3
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA3
• Protein Family: ADP-ribosylation factor-binding protein
• UniProt Gene Name: GGA3
• UniProt Synonym Gene Names: KIAA0154
• UniProt Entry Name: GGA3_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

GGA3: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Monomer. Interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200. Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1 and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Ubiquitously expressed. Belongs to the GGA protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: clathrin adaptor complex; endosome membrane; trans-Golgi network

Molecular Function: ADP-ribosylation factor binding; protein binding

Biological Process: cellular protein metabolic process; intracellular protein transport; positive regulation of protein catabolic process; vesicle-mediated transport