Rabbit anti-Human GFRAL Polyclonal Antibody | anti-GFRAL antibody

GFRAL, CT (GFRAL, C6orf144, GDNF family receptor alpha-like) (FITC)

Cat. Num. AAA6302020

• Gene Names: GFRAL; GRAL; UNQ9356; C6orf144; bA360D14.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GFRAL; Polyclonal Antibody; CT (GFRAL; C6orf144; GDNF family receptor alpha-like) (FITC); GDNF family receptor alpha-like; anti-GFRAL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GFRAL.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GFRAL antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 366-394 in the C-terminal region of human GFRAL.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB435 cell line lysates (35ug/lane) usingMBS6002313.This demonstrates that 036011detected the GFRAL protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6002313 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002313 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB435 cells (right fhistogram) using 036011compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GFRAL antibody

Antibodies; Receptors

References

1. Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009). 2. Li, Z., et al. J. Neurochem. 95(2):361-376(2005). 3. Mungall, A.J., et al. Nature 425(6960):805-811(2003). 4. Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 148231468
• NCBI GeneID: 389400
• NCBI Accession #: NP_997293.2
• NCBI GenBank Nucleotide #: NM_207410.2
• UniProt Accession #: Q6UXV0; Q5VTF6
• Molecular Weight: 44,518 Da
• NCBI Official Full Name: GDNF family receptor alpha-like
• NCBI Official Synonym Full Names: GDNF family receptor alpha like
• NCBI Official Symbol: GFRAL
• NCBI Official Synonym Symbols: GRAL; UNQ9356; C6orf144; bA360D14.1
• NCBI Protein Information: GDNF family receptor alpha-like; IVFI9356
• UniProt Protein Name: GDNF family receptor alpha-like
• Protein Family: GDNF family receptor
• UniProt Gene Name: GFRAL
• UniProt Synonym Gene Names: C6orf144; UNQ9356/PRO34128
• UniProt Entry Name: GFRAL_HUMAN