Rabbit GFAP Polyclonal Antibody | anti-GFAP antibody

Anti-GFAP

Cat. Num. AAA515978

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity chromatography
• Synonyms: GFAP; Polyclonal Antibody; Anti-GFAP; anti-GFAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit, IgG
• Specificity: Recognizes the glial fibrillary acidic protein (GFAP)
• Purity/Purification: Affinity chromatography
• Form/Format: 100 ul neat serum

• Applicable Applications for anti-GFAP antibody: Western Blot (WB), Immunofluorescence (IF)
• Immunogen: Recombinant and purified bovine GFAP.
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; 1 year at -20 degree C from date of shipment

Immunofluorescence (IF)

(Sample Data: Immunofluorescence showing specific labeling of GFAP (red) and vimentin (green) in cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.)

Western Blot (WB)

(Sample Data: Western blot of rat cortex lysate showing specific immunolabeling of the ~50k GFAP protein.)

Related Product Information for anti-GFAP antibody

Glial Fibrillary Acidic Protein (GFAP) was discovered as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).

Product Categories/Family for anti-GFAP antibody

Primary Antibodies; Neurobiology

References

1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972).
2. Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001).