Rabbit anti-Human, Mouse GEMIN5 Polyclonal Antibody | anti-GEMIN5 antibody

GEMIN5 Antibody

Cat. Num. AAA9629812

• Gene Names: GEMIN5; GEMIN-5
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GEMIN5; Polyclonal Antibody; GEMIN5 Antibody; gem (nuclear organelle) associated protein 5; Gem-associated protein 5; anti-GEMIN5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GEMIN5 Antibody detects endogenous levels of GEMIN5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GEMIN5 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GEMIN5.
• Conjugation: Unconjugated
• Similarity: The WD repeat domain mediates binding to U1 snRNA and to U4 snRNA (PubMed:19377484, PubMed:19750007, PubMed:27834343, PubMed:27881600, PubMed:27881601). The WD repeat domain also mediates binding to the 7-methylguanosine cap that is found both on mRNA and snRNA molecules (PubMed:19750007, PubMed:27834343, PubMed:27881600, PubMed:27881601, Ref. 25). The regions that bind snRNA molecules and the isolated 7-methylguanosine cap overlap at least partially (PubMed:27834343, PubMed:27881600, PubMed:27881601). Besides, the WD repeat domain mediates interaction with the 60S large ribosomal subunit (PubMed:27507887). Belongs to the WD repeat gemin-5 family.
• Subunit Structure: Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1, SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE. Identified in a SMN complex that contains GEMIN2/SIP1. Interacts with cytosolic DDX20/GEMIN3 and GEMIN4. Interacts with SNRNP70 and HNRNPU. Identified in a complex with 80S ribosomes; binds to the 60S large ribosomal subunit. Interacts with the ribosomal subunits RPL3 and RPL4.
• Function: Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules Ref.25). Binds to the 3'-UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability. May play a role in the regulation of protein synthesis via its interaction with ribosomes.
• Note: Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-GEMIN5 antibody

Nucleus; Nucleoplasm; Gem; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 356461016
• NCBI GeneID: 25929
• NCBI Accession #: NP_001239085.1
• NCBI GenBank Nucleotide #: NM_001252156.1
• UniProt Accession #: Q8TEQ6; Q14CV0; Q8WWV4; Q969W4; Q9H9K3; Q9UFI5
• Molecular Weight: 168,589 Da
• NCBI Official Full Name: gem-associated protein 5 isoform 2
• NCBI Official Synonym Full Names: gem nuclear organelle associated protein 5
• NCBI Official Symbol: GEMIN5
• NCBI Official Synonym Symbols: GEMIN-5
• NCBI Protein Information: gem-associated protein 5
• UniProt Protein Name: Gem-associated protein 5
• Protein Family: Gem-associated protein
• UniProt Gene Name: GEMIN5
• UniProt Synonym Gene Names: Gemin5
• UniProt Entry Name: GEMI5_HUMAN

NCBI Description

This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]